Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system

被引：18
|
作者：
Maleska, Aleksandra [1 ,2 ,3 ]
Hirtz, Christophe [1 ]
Casteleyn, Elise [1 ]
Villard, Orianne [4 ]
Ducos, Jacques [2 ,3 ]
Avignon, Antoine [4 ]
Sultan, Ariane [4 ]
Lehmann, Sylvain [1 ]
机构：
[1] Univ Montpellier, CHU Montpellier, INSERM U1183, Lab Biochim Proteom Clin, F-34000 Montpellier, France
[2] CHU Montpellier, Hop Lapeyronie, Lab Virol, F-34000 Montpellier, France
[3] INSERM, U1058, F-34000 Montpellier, France
[4] Univ Montpellier, Univ Hosp, CNRS UMR 9214, PhyMedExp,INSERM U1046,Endocrinol Diabetol Nutr D, Montpellier, France
来源：
BIOANALYSIS | 2017年 / 9卷 / 05期
关键词：
clinical biology; DBS; dried blood spot; glycated hemoglobin; HbA1c; HPLC; ion exchange; ISO15189; method validation; DIABETES CONTROL; COMPLICATIONS TRIAL/EPIDEMIOLOGY; HEMOGLOBIN A1C; FILTER-PAPER; INTERVENTIONS; OUTCOMES; GLUCOSE; PATIENT; HBA(1C); SAMPLES;
D O I：
10.4155/bio-2016-0278
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation. Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent. Conclusion: This study provides for the first time a complete method comparison and validation under the ISO15189 guideline using an automated HPLC system. This approach constitutes, therefore, a useful tool for diagnosing diabetes.
引用
收藏
页码：427 / 434
页数：8
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