AN INFLUENZA HEMAGGLUTININ A PEPTIDE ASSAY BASED ON THE ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE

被引：4

作者：

Chiu, May L. ^{[1
]}

Lai, Denton ^{[1
]}

Monbouquette, Harold G. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Chem & Biomol Engn Dept, Los Angeles, CA 90095 USA

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2011年 / 32卷 / 01期

基金：

美国国家卫生研究院;

关键词：

EMIT; enzyme multiplied immunoassay technique; hemagglutinin A; peptide detection; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; BETA-GALACTOSIDASE; LEUCONOSTOC-MESENTEROIDES; HOMOGENEOUS IMMUNOASSAYS; PROTEIN; SENSITIVITY; OPIATES; EMIT;

D O I：

10.1080/15321819.2011.538623

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A practical approach for constructing enzyme-multiplied immunoassay technique (EMIT)-based protein/ peptide assays is described. Normally used in small-molecule drug testing, EMIT is a homogeneous assay method that is attractive for its simplicity, sensitivity, and rapidity. The EMIT-based peptide/ protein assay was developed by conjugating a cysteine-modified HA peptide (from influenza hemagglutinin A) to the reporter enzyme, glucose-6-phosphate dehydrogenase. The 13-min assay gave a free HA limit of detection of 10 nM and proved effective for detection of a high-molecular-weight model protein tagged with HA. Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection.

引用

页码：1 / 17

页数：17

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