AN INFLUENZA HEMAGGLUTININ A PEPTIDE ASSAY BASED ON THE ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE

被引：4
|
作者：
Chiu, May L. [1 ]
Lai, Denton [1 ]
Monbouquette, Harold G. [1 ]
机构：
[1] Univ Calif Los Angeles, Chem & Biomol Engn Dept, Los Angeles, CA 90095 USA
来源：
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2011年 / 32卷 / 01期
基金：
美国国家卫生研究院;
关键词：
EMIT; enzyme multiplied immunoassay technique; hemagglutinin A; peptide detection; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; BETA-GALACTOSIDASE; LEUCONOSTOC-MESENTEROIDES; HOMOGENEOUS IMMUNOASSAYS; PROTEIN; SENSITIVITY; OPIATES; EMIT;
D O I：
10.1080/15321819.2011.538623
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A practical approach for constructing enzyme-multiplied immunoassay technique (EMIT)-based protein/ peptide assays is described. Normally used in small-molecule drug testing, EMIT is a homogeneous assay method that is attractive for its simplicity, sensitivity, and rapidity. The EMIT-based peptide/ protein assay was developed by conjugating a cysteine-modified HA peptide (from influenza hemagglutinin A) to the reporter enzyme, glucose-6-phosphate dehydrogenase. The 13-min assay gave a free HA limit of detection of 10 nM and proved effective for detection of a high-molecular-weight model protein tagged with HA. Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection.
引用
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页码：1 / 17
页数：17
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