Rapid and simultaneous determination of 25-OH-vitamin D-2 and D-3 in human serum by LC/MS/MS: Validation and uncertainty assessment

被引：0
|
作者：
Goren, Ahmet C. [1 ]
Bilsel, Gokhan [1 ]
Bilsel, Mine [1 ]
机构：
[1] TUBITAK, UME, P Box 54, TR-41470 Gebze, Turkey
来源：
JOURNAL OF CHEMICAL METROLOGY | 2007年 / 1卷 / 01期
关键词：
25-OH Vitamin D-3; 25-OH Vitamin D-2; LC/MS/MS; method validation; uncertainty;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
25-OH Vitamin D-3 and 25-OH Vitamin D-2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D-3 and 25-OH Vitamin D-2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 mu g L-1 concentration averaged to 92 % for 25-OH vitamin D-3 and 94 % for 25-OH-vitamin D-2. The uncertainty of the measurements for 25-OH vitamin D-3 and for 25-OH-vitamin D-2 was 7.77 % and 7.24 %, respectively.
引用
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页码：1 / 9
页数：9
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