Rapid and simultaneous determination of 25-OH-vitamin D-2 and D-3 in human serum by LC/MS/MS: Validation and uncertainty assessment

被引：0
|
作者：
Goren, Ahmet C. [1 ]
Bilsel, Gokhan [1 ]
Bilsel, Mine [1 ]
机构：
[1] TUBITAK, UME, P Box 54, TR-41470 Gebze, Turkey
来源：
JOURNAL OF CHEMICAL METROLOGY | 2007年 / 1卷 / 01期
关键词：
25-OH Vitamin D-3; 25-OH Vitamin D-2; LC/MS/MS; method validation; uncertainty;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
25-OH Vitamin D-3 and 25-OH Vitamin D-2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D-3 and 25-OH Vitamin D-2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 mu g L-1 concentration averaged to 92 % for 25-OH vitamin D-3 and 94 % for 25-OH-vitamin D-2. The uncertainty of the measurements for 25-OH vitamin D-3 and for 25-OH-vitamin D-2 was 7.77 % and 7.24 %, respectively.
引用
收藏
页码：1 / 9
页数：9
相关论文
共 50 条
  • [11] Simultaneous quantification of vitamin D3, 25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 in human serum by LC-MS/MS
    Burild, Anders
    Frandsen, Henrik L.
    Jakobsen, Jette
    SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2014, 74 (05): : 418 - 423
  • [12] Development and validation of the simultaneous measurement of four vitamin D metabolites in serum by LC–MS/MS for clinical laboratory applications
    Mamoru Satoh
    Takayuki Ishige
    Shoujiro Ogawa
    Motoi Nishimura
    Kazuyuki Matsushita
    Tatsuya Higashi
    Fumio Nomura
    Analytical and Bioanalytical Chemistry, 2016, 408 : 7617 - 7627
  • [13] Development and validation of an LC-MS/MS based method for quantification of 25 hydroxyvitamin D2 and 25 hydroxyvitamin D3 in human serum and plasma
    Zhang, Stanley
    Jian, Wenying
    Sullivan, Sheryl
    Sankaran, Banu
    Edom, Richard W.
    Weng, Naidong
    Sharkey, David
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 961 : 62 - 70
  • [14] Simultaneous determination of vitamins A, E and 25-OH-vitamin D:: Application in clinical assessments
    Granado-Lorencio, F
    Olmedilla-Alonso, B
    Herrero-Barbudo, C
    Blanco-Navarro, I
    Blázquez-García, S
    Pérez-Sacristán, B
    CLINICAL BIOCHEMISTRY, 2006, 39 (02) : 180 - 182
  • [15] A rapid method for simultaneous measurements of 25-hydroxyvitamins D2 and D3 employing LC-MS/MS
    Law, T.
    Suzuki, K.
    Polio, S.
    Kellogg, M.
    CLINICAL CHEMISTRY, 2008, 54 (06) : A141 - A141
  • [16] Observation of a positive interference in LC-MS/MS measurement of d6-25-OH-vitamin D3
    Fortuna, Danielle
    Korn, Warren R.
    Brune, Matthew J.
    He, Xiang
    Wang, Alexandre Y.
    Hevko, John M.
    Stickle, Douglas F.
    CLINICAL MASS SPECTROMETRY, 2017, 3 : 22 - 24
  • [17] LC-MS/MS analysis of 25 OH vitamin D2 and D3 compared to the DiaSorin LIAISION® and RIA methods
    Fenske, JS
    Pieper, KA
    Belisle, KJ
    Eastvold, M
    Singh, RJ
    CLINICAL CHEMISTRY, 2005, 51 : A114 - A115
  • [18] Response to '25-OH Vitamin D concentrations measured by LC-MS/MS are equivalent in serum and EDTA plasma'
    Jensen, M. E.
    Murphy, V. E.
    Harvey, S.
    Gibson, P. G.
    Clarke, M.
    STEROIDS, 2022, 187
  • [19] Comparison of diasorin methods for measurement of 25 OH vitamin D with the LC-MS/MS method
    Fenske, JS
    Pieper, KA
    Belisle, KJ
    Eastvold, M
    Singh, RJ
    CLINICA CHIMICA ACTA, 2005, 355 : S256 - S256
  • [20] Vitamin D and 25-hydroxyvitamin D determination in meats by LC-IT-MS
    Strobel, Norbert
    Buddhadasa, Saman
    Adorno, Paul
    Stockham, Katherine
    Greenfield, Heather
    FOOD CHEMISTRY, 2013, 138 (2-3) : 1042 - 1047
12345