Rapid and simultaneous determination of 25-OH-vitamin D-2 and D-3 in human serum by LC/MS/MS: Validation and uncertainty assessment

被引：0

作者：

Goren, Ahmet C. ^{[1
]}

Bilsel, Gokhan ^{[1
]}

Bilsel, Mine ^{[1
]}

机构：

[1] TUBITAK, UME, P Box 54, TR-41470 Gebze, Turkey

来源：

JOURNAL OF CHEMICAL METROLOGY | 2007年 / 1卷 / 01期

关键词：

25-OH Vitamin D-3; 25-OH Vitamin D-2; LC/MS/MS; method validation; uncertainty;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

25-OH Vitamin D-3 and 25-OH Vitamin D-2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D-3 and 25-OH Vitamin D-2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 mu g L-1 concentration averaged to 92 % for 25-OH vitamin D-3 and 94 % for 25-OH-vitamin D-2. The uncertainty of the measurements for 25-OH vitamin D-3 and for 25-OH-vitamin D-2 was 7.77 % and 7.24 %, respectively.

引用

页码：1 / 9

页数：9

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