Imaging the biogenesis of individual HIV-1 virions in live cells

被引：0
|
作者：
Nolwenn Jouvenet
Paul D. Bieniasz
Sanford M. Simon
机构：
[1] Aaron Diamond AIDS Research Center,
[2] ,undefined
[3] Laboratory of Retrovirology,undefined
[4] and,undefined
[5] ,undefined
[6] Laboratory of Cellular Biophysics,undefined
[7] The Rockefeller University,undefined
[8] New York,undefined
[9] New York 10065,undefined
[10] USA,undefined
来源：
Nature | 2008年 / 454卷
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摘要：
For the first time, the assembly of a virus particle has been observed directly in live cells. Using techniques based on total internal reflection fluorescence microscopy, and live HeLa cells expressing fluorescent protein-tagged derivatives of Gag, the major structural component of HIV-1, individual HIV-1 virus particles were observed in real time. The resulting movies resolve a controversy over where in the cells HIV-1 assembly occurs — the virions appear individually at the plasma membrane and are assembled in about 5 or 6 minutes.
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页码：236 / 240
页数：4
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