Imaging the biogenesis of individual HIV-1 virions in live cells

被引：0
|
作者：
Nolwenn Jouvenet
Paul D. Bieniasz
Sanford M. Simon
机构：
[1] Aaron Diamond AIDS Research Center,
[2] ,undefined
[3] Laboratory of Retrovirology,undefined
[4] and,undefined
[5] ,undefined
[6] Laboratory of Cellular Biophysics,undefined
[7] The Rockefeller University,undefined
[8] New York,undefined
[9] New York 10065,undefined
[10] USA,undefined
来源：
Nature | 2008年 / 454卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
For the first time, the assembly of a virus particle has been observed directly in live cells. Using techniques based on total internal reflection fluorescence microscopy, and live HeLa cells expressing fluorescent protein-tagged derivatives of Gag, the major structural component of HIV-1, individual HIV-1 virus particles were observed in real time. The resulting movies resolve a controversy over where in the cells HIV-1 assembly occurs — the virions appear individually at the plasma membrane and are assembled in about 5 or 6 minutes.
引用
收藏
页码：236 / 240
页数：4
相关论文
共 50 条
  • [31] Vaginal Langerhans cells resist genomic integration of HIV-1 but transmit endocytosed virions to susceptible target cells
    Robinson, Barry R.
    Ballweber, Lamar
    Kreger, Allison
    Lentz, Gretchen M.
    Fialkow, Michael F.
    McElrath, M. Juliana
    Hladik, Florian
    JOURNAL OF IMMUNOLOGY, 2009, 182
  • [32] Imaging of individual signaling molecules in live cells
    Snaar-Jagalska, BE
    Lommerse, PHM
    de Keijzer, S
    Blab, GA
    Spaink, HP
    Schmidt, T
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 403A - 403A
  • [33] Paradigm Shift in the Mechanism of HIV-1 Core Biogenesis
    Frank, Gabriel A.
    Narayan, Kedar
    Bess, Julian W., Jr.
    Del Prete, Gregory Q.
    Wu, Xiongwu
    Moran, Amy
    Hartnell, Lisa
    Earl, Lesley A.
    Lifson, Jeffrey D.
    Subramaniam, Sriram
    BIOPHYSICAL JOURNAL, 2015, 108 (02) : 527A - 527A
  • [34] Glutathionylated forms of the HIV-1 protease are substrates for human thioltransferase (glutaredoxin), an enzyme also detected in HIV-1 virions
    Davis, DA
    Newcomb, FM
    Starke, DW
    Ott, DE
    Mieyal, JJ
    Yarchoan, R
    FASEB JOURNAL, 1997, 11 (09): : A982 - A982
  • [35] HIV-1 assembly: Viral glycoproteins segregate quantally to lipid rafts that associate individually with HIV-1 capsids and virions
    Leung, Kwanyee
    Kim, Jae-Ouk
    Ganesh, Lakshmanan
    Kabat, Juraj
    Schwartz, Owen
    Nabel, Gary J.
    CELL HOST & MICROBE, 2008, 3 (05) : 285 - 292
  • [36] MONOCLONAL-ANTIBODIES THAT NEUTRALIZE HIV-1 VIRIONS AND INHIBIT SYNCYTIUM FORMATION BY INFECTED-CELLS
    FUNG, MSC
    SUN, C
    SUN, NC
    CHANG, NT
    CHANG, TW
    BIO-TECHNOLOGY, 1987, 5 (09): : 940 - &
  • [37] The use of nanoparticle technology in capturing HIV-1 virions and viral proteins
    Jaworski, Elizabeth
    Shafagati, Nazly
    Van Duyne, Rachel
    Saifuddin, Mohammed
    Iordanskiy, Sergey
    Kehn-Hall, Kylene
    Lepene, Benjamin
    Kashanchi, Fatah
    JOURNAL OF NEUROVIROLOGY, 2013, 19 : S40 - S40
  • [38] Retromer Regulates HIV-1 Envelope Glycoprotein Trafficking and Incorporation into Virions
    Groppelli, Elisabetta
    Len, Alice C.
    Granger, Luke A.
    Jolly, Clare
    PLOS PATHOGENS, 2014, 10 (11)
  • [39] The Use of Nanotrap Particles Technology in Capturing HIV-1 Virions and Viral Proteins from Infected Cells
    Jaworski, Elizabeth
    Saifuddin, Mohammed
    Sampey, Gavin
    Shafagati, Nazly
    Van Duyne, Rachel
    Iordanskiy, Sergey
    Kehn-Hall, Kylene
    Liotta, Lance
    Petricoin, Emanuel, III
    Young, Mary
    Lepene, Benjamin
    Kashanchi, Fatah
    PLOS ONE, 2014, 9 (05):
  • [40] Comparing methods to detect cellular proteins on the surface of HIV-1 virions
    Chaphekar, Deepa
    Fernandes, Claire
    Persaud, Arvin T.
    Guzzo, Christina
    JOURNAL OF VIROLOGICAL METHODS, 2025, 333
12345