Single objective tilted lightsheet for three-dimensional localization microscopy

被引：0
|
作者：
Hung, Shih-Te [1 ]
Cnossen, Jelmer [1 ]
Fan, Daniel [1 ]
Smith, Carlas S. [1 ]
机构：
[1] Delft Univ Technol, Delft Ctr Syst & Control, Delft, Netherlands
来源：
ADVANCES IN MICROSCOPIC IMAGING III | 2021年 / 11922卷
关键词：
D O I：
10.1117/12.2615803
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution. (C) 2021 The Author(s)
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页数：2
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