Single objective tilted lightsheet for three-dimensional localization microscopy

被引：0
|
作者：
Hung, Shih-Te [1 ]
Cnossen, Jelmer [1 ]
Fan, Daniel [1 ]
Smith, Carlas S. [1 ]
机构：
[1] Delft Univ Technol, Delft Ctr Syst & Control, Delft, Netherlands
来源：
ADVANCES IN MICROSCOPIC IMAGING III | 2021年 / 11922卷
关键词：
D O I：
10.1117/12.2615803
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution. (C) 2021 The Author(s)
引用
收藏
页数：2
相关论文
共 50 条
  • [21] Single-shot afocal three-dimensional microscopy
    Feldkhun, Daniel
    Wagner, Kelvin H.
    OPTICS LETTERS, 2016, 41 (15) : 3483 - 3486
  • [22] Three-dimensional spectroscopic single-molecule localization microscopy using bi-plane detection
    Song, Ki-Hee
    Zhang, Yang
    Sun, Cheng
    Zhang, Hao
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 257
  • [23] Subwavelength three-dimensional Rydberg atom localization by optical absorption microscopy
    Zhang, Hengfei
    Yuan, Jinpeng
    Wu, Chaohua
    Wang, Lirong
    Xiao, Liantuan
    Jia, Suotang
    LASER PHYSICS LETTERS, 2021, 18 (01)
  • [24] 10 nm three-dimensional localization using Bessel beam microscopy
    Chakraborty, Chumki
    Islam, Md Anisul
    Snoeyink, Craig
    MEASUREMENT SCIENCE AND TECHNOLOGY, 2020, 31 (04)
  • [25] Three-dimensional indoor visible light positioning system with a single transmitter and a single tilted receiver
    Li, Qing-Lin
    Wang, Jin-Yuan
    Huang, Ting
    Wang, Yongjin
    OPTICAL ENGINEERING, 2016, 55 (10)
  • [26] Imaging three-dimensional single molecule orientations by defocused microscopy
    Wang, Jinyuan
    Wang, Chen
    Liu, Li
    Wang, Guiying
    Guangxue Xuebao/Acta Optica Sinica, 2007, 27 (05): : 766 - 770
  • [27] Electrochemistry of Single Nanodomains Revealed by Three-Dimensional Holographic Microscopy
    Brasiliense, Vitor
    Berto, Pascal
    Combellas, Catherine
    Tessier, Gilles
    Kanoufi, Frederic
    ACCOUNTS OF CHEMICAL RESEARCH, 2016, 49 (09) : 2049 - 2057
  • [28] Three-dimensional localization of sperm whales using a single hydrophone
    Tiemann, Christopher O.
    Thode, Aaron M.
    Straley, Janice
    O'Connell, Victoria
    Folkert, Kendall
    Journal of the Acoustical Society of America, 2006, 120 (04): : 2355 - 2365
  • [29] Three-dimensional localization of sperm whales using a single hydrophone
    Tiemann, Christopher O.
    Thode, Aaron M.
    Straley, Janice
    O'Connell, Victoria
    Folkert, Kendall
    JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA, 2006, 120 (04): : 2355 - 2365
  • [30] Three-dimensional nano-localization of single fluorescent emitters
    Maerki, Iwan
    Bocchio, Noelia L.
    Geissbuehler, Stefan
    Aguet, Francois
    Bilenca, Alberto
    Lasser, Theo
    OPTICS EXPRESS, 2010, 18 (19): : 20263 - 20272
12345