Blunt-ended DNA stacking interactions in a 3-helix motif

被引：34
|
作者：
Wang, Risheng [1 ]
Kuzuya, Akinori [2 ]
Liu, Wenyan [1 ]
Seeman, Nadrian C. [1 ]
机构：
[1] NYU, Dept Chem, New York, NY 10003 USA
[2] Univ Tokyo, Res Ctr Adv Sci & Technol, Meguro Ku, Tokyo 1538904, Japan
来源：
CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 27期
基金：
美国国家科学基金会;
关键词：
CRYSTALS; MOLECULE; PROTEIN; DESIGN;
D O I：
10.1039/c0cc01167c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We demonstrate that intermolecular stacking is capable of forming one-dimensional arrays of a blunt-ended 3-helix DNA motif. The array can be visualized in the atomic force microscopy through conjugated streptavidin nanoparticles. We estimate the strength of the triple stacking interaction to be -8.6 kcal mol(-1).
引用
收藏
页码：4905 / 4907
页数：3
相关论文
共 33 条
  • [11] TURBO CLONING - A FAST, EFFICIENT METHOD FOR CLONING PCR PRODUCTS AND OTHER BLUNT-ENDED DNA FRAGMENTS INTO PLASMIDS
    BOYD, AC
    NUCLEIC ACIDS RESEARCH, 1993, 21 (04) : 817 - 821
  • [12] Visualization of Irreparable Ischemic Damage in Brain by Selective Labeling of Double-Strand Blunt-Ended DNA Breaks
    Vladimir V. Didenko
    Hop Ngo
    Candace L. Minchew
    Denise J. Boudreaux
    Marsha A. Widmayer
    David S. Baskin
    Molecular Medicine, 2002, 8 : 818 - 823
  • [13] A MODIFIED PBR322 VECTOR WITH IMPROVED PROPERTIES FOR THE CLONING, RECOVERY, AND SEQUENCING OF BLUNT-ENDED DNA FRAGMENTS
    PRENTKI, P
    KRISCH, HM
    GENE, 1982, 17 (02) : 189 - 196
  • [14] Comparing 2-nt 3′ overhangs against blunt-ended siRNAs: a systems biology based study
    Ghosh, Preetam
    Dullea, Robert
    Fischer, James E.
    Turi, Tom G.
    Sarver, Ronald W.
    Zhang, Chaoyang
    Basu, Kalyan
    Das, Sajal K.
    Poland, Bradley W.
    BMC GENOMICS, 2009, 10
  • [15] HIV-1 lntegrase Strand Transfer Inhibitors Stabilize an Integrase-Single Blunt-Ended DNA Complex
    Bera, Sibes
    Pandey, Krishan K.
    Vora, Ajaykumar C.
    Grandgenett, Duane P.
    JOURNAL OF MOLECULAR BIOLOGY, 2011, 410 (05) : 831 - 846
  • [16] Comparing 2-nt 3' overhangs against blunt-ended siRNAs: a systems biology based study
    Preetam Ghosh
    Robert Dullea
    James E Fischer
    Tom G Turi
    Ronald W Sarver
    Chaoyang Zhang
    Kalyan Basu
    Sajal K Das
    Bradley W Poland
    BMC Genomics, 10
  • [17] Archaeal DNA helicase HerA interacts with Mre11 homologue and unwinds blunt-ended double-stranded DNA and recombination intermediates
    Zhang, Songtao
    Wei, Tao
    Hou, Guihua
    Zhang, Chao
    Liang, Penjuan
    Ni, Jinfeng
    Sheng, Duohong
    Shen, Yulong
    DNA REPAIR, 2008, 7 (03) : 380 - 391
  • [18] Blunt-ended DNA double-strand breaks induced by endonucleases PvuII and EcoRV are poor substrates for repair in Saccharomyces cerevisiae
    Westmoreland, James W.
    Summers, Jennifer A.
    Holland, Cory L.
    Resnick, Michael A.
    Lewis, L. Kevin
    DNA REPAIR, 2010, 9 (06) : 617 - 626
  • [19] A QuikChange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously
    An, Yingfeng
    Lv, Anguo
    Wu, Wenfang
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 397 (02) : 136 - 139
  • [20] REMOVAL OF 3'-OH-TERMINAL NUCLEOTIDES FROM BLUNT-ENDED LONG TERMINAL REPEAT TERMINI BY THE AVIAN RETROVIRUS INTEGRATION PROTEIN
    VORA, AC
    FITZGERALD, ML
    GRANDGENETT, DP
    JOURNAL OF VIROLOGY, 1990, 64 (11) : 5656 - 5659
1234