Integrated sample injection chip for Micro-HPLC

被引：0
|
作者：
Ezaki, Takahiro [1 ,2 ]
Yamazaki, Takeo [2 ]
Yasuda, Susumu [2 ]
Yagi, Takayuki [2 ]
机构：
[1] Canon Inc, Ohta Ku, 30-2 Shimomaruko 3 Chome, Tokyo 1468501, Japan
[2] CANON Inc, Canon Res Ctr, NEMS Platform Res Div, Tokyo, Japan
来源：
2006 1ST IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3 | 2006年
关键词：
sample injection; microvalve; passive valve; low pass valve; HPLC;
D O I：
10.1109/NEMS.2006.334578
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
In this paper we present a novel sample injection chip. Microvalves and microchannels are integrated on the chip. The valve is a passive lowpass valve that allows a liquid flow at a low flow rate and prohibits it when the flow rate exceeds a threshold. We can control the injection volume by changing the channel design. The chip consists of a channel substrate, an upper valve substrate and an under valve substrate. These substrates are separately fabricated and bonded by using MEMS techniques. The chip, not to leak at a pressure difference of 5.0MPa, was successfully applied to high performance liquid chromatography (HPLC). A specific volume of sample (100 nl) was injected to the HPLC column. The results show that the sample injection chip has the possibility of being used in the micro-HPLC systems.
引用
收藏
页码：979 / +
页数：2
相关论文
共 50 条
  • [31] THE USE OF MICRO-HPLC WITH GRADIENT ELUTION FOR THE CHARACTERIZATION OF PHENOL-FORMALDEHYDE RESINS
    PREUSSLER, V
    SLAIS, K
    HANUS, J
    ANGEWANDTE MAKROMOLEKULARE CHEMIE, 1987, 150 : 179 - 187
  • [32] UTILIZATION OF MICRO-HPLC TECHNIQUE FOR ESTIMATING HYDROPHOBIC SUBSTITUENT PARAMETERS IN QSAR WORKS
    JINNO, K
    ANALYTICAL LETTERS PART A-CHEMICAL ANALYSIS, 1982, 15 (19): : 1533 - 1537
  • [33] Micro-HPLC and standard-size HPLC for the separation of peptide stereoisomers employing an ion-exchange principle
    Czerwenka, C
    Lämmerhofer, M
    Lindner, W
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 30 (06) : 1789 - 1800
  • [34] SEPARATION AND DETERMINATION OF PURPUREA GLYCOSIDES IN DIGITALIS-PURPUREA LEAVES BY MICRO-HPLC
    FUJII, Y
    IKEDA, Y
    YAMAZAKI, M
    JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1987, 10 (03): : 137 - 140
  • [35] Optimization of micro-HPLC peak focusing for the detection and quantification of low hepcidin concentrations
    Mansion, Francois
    Chiap, Patrice
    Houbart, Virginie
    Crommen, Jacques
    Servais, Anne-Catherine
    Fillet, Marianne
    JOURNAL OF SEPARATION SCIENCE, 2011, 34 (15) : 1820 - 1827
  • [36] VERY SHORT MICRO-HPLC COLUMN PACKED WITH 3-MICRON PARTICLES
    JINNO, K
    ANALYTICAL LETTERS PART A-CHEMICAL ANALYSIS, 1984, 17 (10): : 933 - 943
  • [37] Enantioseparation of dipeptides and tripeptides by micro-HPLC comparing teicoplanin and teicoplanin aglycone as chiral selectors
    Schmid, MG
    Hölbling, M
    Schnedlitz, N
    Gübitz, G
    JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2004, 61 (1-2): : 1 - 10
  • [38] Micro analysis system for pH and protease activities with an integrated sample injection mechanism
    Morimoto, Katsuya
    Suzuki, Hiroaki
    BIOSENSORS & BIOELECTRONICS, 2006, 22 (01): : 86 - 93
  • [39] Microfluidic chip for peptide analysis with an integrated HPLC column, sample enrichment column, and nanoelectrospray tip
    Yin, NF
    Killeen, K
    Brennen, R
    Sobek, D
    Werlich, M
    van de Goor, TV
    ANALYTICAL CHEMISTRY, 2005, 77 (02) : 527 - 533
  • [40] THE USE OF CAPACITY FACTORS WITH MICRO-HPLC AS A DESCRIPTORS IN QUANTITATIVE-STRUCTURE-ACTIVITY RELATIONSHIPS
    JINNO, K
    ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1984, 17 (03): : 183 - 190
12345