HPLC-API/MS/MS: a powerful tool for integrating drug metabolism into the drug discovery process

被引：91
|
作者：
Korfmacher, WA [1 ]
Cox, KA [1 ]
Bryant, MS [1 ]
Veals, J [1 ]
Ng, K [1 ]
Watkins, R [1 ]
Lin, CC [1 ]
机构：
[1] SCHERING PLOUGH CORP,RES INST,DEPT PHARMACOL,KENILWORTH,NJ 07033
来源：
DRUG DISCOVERY TODAY | 1997年 / 2卷 / 12期
关键词：
D O I：
10.1016/S1359-6446(97)01121-5
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
HPLC combined with atmospheric pressure ionization (API) mass spectrometry (MS) has become a very useful tool in the pharmaceutical industry. The technique of HPLC-API/MS/MS is becoming very important for both drug discovery and drug development programs. In the drug discovery area, it has three major uses: (1) rapid, quantitative method development, (2) metabolite identification, and (3) multi-drug analysis. The sensitivity of the API source and the selectivity provided by tandem mass spectrometry (MS/MS) enable rapid, quantitative method development for drugs in plasma. Early information on the metabolism of candidate drugs can guide structural modifications, thereby improving the activity and/or bioavailability.
引用
收藏
页码：532 / 537
页数：10
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