Positively coded photoaffinity label for altering isoelectric points of proteins

被引:1
|
作者
Hashimoto, Makoto
Hatanaka, Yasumaru
机构
[1] Obihiro Univ Agr & Vet Med, Dept Agr & Life Sci, Obihiro, Hokkaido 0808555, Japan
[2] Toyama Univ, Fac Pharmaceut Sci, Toyama 9300194, Japan
关键词
photoaffinity labeling; diazirine; isoelectric focusing; biotin;
D O I
10.1016/j.bmcl.2006.08.119
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5998 / 6000
页数:3
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