Positively coded photoaffinity label for altering isoelectric points of proteins

被引：1
|
作者：
Hashimoto, Makoto
Hatanaka, Yasumaru
机构：
[1] Obihiro Univ Agr & Vet Med, Dept Agr & Life Sci, Obihiro, Hokkaido 0808555, Japan
[2] Toyama Univ, Fac Pharmaceut Sci, Toyama 9300194, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 23期
关键词：
photoaffinity labeling; diazirine; isoelectric focusing; biotin;
D O I：
10.1016/j.bmcl.2006.08.119
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5998 / 6000
页数：3
相关论文
共 50 条
  • [21] SYNTHESIS OF AZIDOTUBULIN - A PHOTOAFFINITY LABEL FOR TUBULIN-BINDING PROTEINS
    BALCZON, RD
    BRINKLEY, BR
    BIOCHEMISTRY, 1989, 28 (21) : 8490 - 8496
  • [22] PREPARATION OF AZIDOCALMODULIN - A PHOTOAFFINITY LABEL FOR CALMODULIN-BINDING PROTEINS
    ANDREASEN, TJ
    KELLER, CH
    LAPORTE, DC
    EDELMAN, AM
    STORM, DR
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (05): : 2782 - 2785
  • [23] Estimation of isoelectric points of human plasma proteins employing capillary isoelectric focusing and peptide isoelectric point markers
    Jin, Y
    Luo, GA
    Oka, T
    Manabe, T
    ELECTROPHORESIS, 2002, 23 (19) : 3385 - 3391
  • [24] CALCULATION OF THE ISOELECTRIC POINTS OF NATIVE PROTEINS WITH SPREADING OF PK(A) VALUES
    HENRIKSSON, G
    ENGLUND, AK
    JOHANSSON, G
    LUNDAHL, P
    ELECTROPHORESIS, 1995, 16 (08) : 1377 - 1380
  • [25] RELATIONSHIP BETWEEN INVIVO DEGRADATIVE RATES AND ISOELECTRIC POINTS OF PROTEINS
    DICE, JF
    GOLDBERG, AL
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) : 3893 - 3897
  • [26] Ion-exchange chromatography of proteins near the isoelectric points
    Yamamoto, S
    Ishihara, T
    JOURNAL OF CHROMATOGRAPHY A, 1999, 852 (01) : 31 - 36
  • [27] Gradient-free determination of isoelectric points of proteins on chip
    Lapinska, Urszula
    Saar, Kadi L.
    Yates, Emma V.
    Herling, Therese W.
    Muller, Thomas
    Challa, Pavan K.
    Dobson, Christopher M.
    Knowles, Tuomas P. J.
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2017, 19 (34) : 23060 - 23067
  • [28] ELECTROPHORESIS OF RIBONUCLEASE AND OF BETA-LACTOGLOBULIN - ISOELECTRIC POINTS OF PROTEINS
    BARNETT, LB
    BULL, HB
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1960, 89 (02) : 167 - 172
  • [29] The effect of formaldehyde on the isoelectric points of some proteins, determined by microelectrophoresis
    Circle, SJ
    Smith, AK
    JOURNAL OF PHYSICAL CHEMISTRY, 1941, 45 (06): : 916 - 930
  • [30] pIPredict: A computer tool for prediction of isoelectric points of peptides and proteins
    Skvortsov V.S.
    Alekseytchuk N.N.
    Khudyakov D.V.
    Romero Reyes I.V.
    Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2015, 9 (3) : 296 - 303
12345