Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor

被引：7
|
作者：
Zhong, JQ
Freyzon, Y
Ehrlich, DJ
Matsudaira, P [1 ]
机构：
[1] MIT, Dept Biol, Cambridge Ctr 9, Cambridge, MA 02142 USA
[2] MIT, Div Biol Engn, Cambridge Ctr 9, Cambridge, MA 02142 USA
[3] MIT, Whitehead Inst Biomed Res, Cambridge Ctr 9, Cambridge, MA 02142 USA
来源：
BIOMOLECULAR ENGINEERING | 2004年 / 21卷 / 02期
关键词：
green fluorescent protein; affinity fluorescent protein; enhanced fluorescence intensity; solid-phase assay; biosensor;
D O I：
10.1016/j.bioeng.2003.10.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface. (C) 2004 Elsevier B.V. All rights reserved.
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页码：67 / 72
页数：6
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