Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor

被引：7

作者：

Zhong, JQ

Freyzon, Y

Ehrlich, DJ

Matsudaira, P ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge Ctr 9, Cambridge, MA 02142 USA

[2] MIT, Div Biol Engn, Cambridge Ctr 9, Cambridge, MA 02142 USA

[3] MIT, Whitehead Inst Biomed Res, Cambridge Ctr 9, Cambridge, MA 02142 USA

来源：

BIOMOLECULAR ENGINEERING | 2004年 / 21卷 / 02期

关键词：

green fluorescent protein; affinity fluorescent protein; enhanced fluorescence intensity; solid-phase assay; biosensor;

D O I：

10.1016/j.bioeng.2003.10.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：67 / 72

页数：6

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