Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor

被引：7

作者：

Zhong, JQ

Freyzon, Y

Ehrlich, DJ

Matsudaira, P ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge Ctr 9, Cambridge, MA 02142 USA

[2] MIT, Div Biol Engn, Cambridge Ctr 9, Cambridge, MA 02142 USA

[3] MIT, Whitehead Inst Biomed Res, Cambridge Ctr 9, Cambridge, MA 02142 USA

来源：

BIOMOLECULAR ENGINEERING | 2004年 / 21卷 / 02期

关键词：

green fluorescent protein; affinity fluorescent protein; enhanced fluorescence intensity; solid-phase assay; biosensor;

D O I：

10.1016/j.bioeng.2003.10.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：67 / 72

页数：6

共 50 条

[31] MULTIPLE ANTIGEN PEPTIDE - A NOVEL-APPROACH TO INCREASE DETECTION SENSITIVITY OF SYNTHETIC PEPTIDES IN SOLID-PHASE IMMUNOASSAYS
TAM, JP
ZAVALA, F
JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 124 (01) : 53 - 61
[32] Reagents and methods for the solid-phase synthesis of protein-EDTA for use in affinity cleaving
1600, (112):
[33] Sensitive Detection of Transcription Factors Using Near-Infrared Fluorescent Solid-Phase Rolling Circle Amplification
Yin, Junhuan
Gan, Ping
Zhou, Fei
Wang, Jinke
ANALYTICAL CHEMISTRY, 2014, 86 (05) : 2572 - 2579
[34] SENSITIVITY OF DIRECT SOLID-PHASE RADIOIMMUNOASSAY FOR DETECTION OF HEPATITIS-ASSOCIATED ANTIGEN
JENNINGS, ER
DEPRATTI, V
MONROE, CH
POLLACK, W
WATSONWI.EJ
CLAUSS, B
LANCET, 1973, 2 (7831): : 734 - 735
[35] SOLID-PHASE RADIOIMMUNOASSAY FOR DETECTION OF ANTIBODIES TO MYELIN BASIC-PROTEIN
RANDOLPH, DH
KIBLER, RF
FRITZ, RB
JOURNAL OF IMMUNOLOGICAL METHODS, 1977, 18 (3-4) : 215 - 224
[36] Detection and Quantification of Bacteria and Fungi Using Solid-Phase Cytometry
Vanhee, Lies M. E.
D'Haese, Eva
Cools, Ils
Nelis, Hans J.
Coenye, Tom
DETECTION OF BACTERIA, VIRUSES, PARASITES AND FUNGI: BIOTERRORISM PREVENTION, 2010, : 25 - 41
[37] Solid-Phase Extraction and Simultaneous Fluorescence Detection of Cis-Diol-Containing Biomolecules Based on a Novel Boronate Affinity Material
Qianjin Li
Tingting Wang
Yadan Hou
Dan Wang
Shuqing Xiong
Chenhong Wei
Qianyong Cao
Fenying Wang
Chromatographia, 2023, 86 : 387 - 399
[38] Solid-Phase Extraction and Simultaneous Fluorescence Detection of Cis-Diol-Containing Biomolecules Based on a Novel Boronate Affinity Material
Li, Qianjin
Wang, Tingting
Hou, Yadan
Wang, Dan
Xiong, Shuqing
Wei, Chenhong
Cao, Qianyong
Wang, Fenying
CHROMATOGRAPHIA, 2023, 86 (05) : 387 - 399
[39] Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation
Cotton, GJ
Muir, TW
CHEMISTRY & BIOLOGY, 2000, 7 (04): : 253 - 261
[40] A HPLC-BASED DETECTION METHOD FOR FLUORESCENT SEA-WATER TRACERS USING ONLINE SOLID-PHASE EXTRACTION
SUIJLEN, JM
STAAL, W
HOUPT, PM
DRAAIER, A
CONTINENTAL SHELF RESEARCH, 1994, 14 (13-14) : 1523 - 1538

← 1 2 3 4 5 →