AN INFLUENZA HEMAGGLUTININ A PEPTIDE ASSAY BASED ON THE ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE

被引：4

作者：

Chiu, May L. ^{[1
]}

Lai, Denton ^{[1
]}

Monbouquette, Harold G. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Chem & Biomol Engn Dept, Los Angeles, CA 90095 USA

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2011年 / 32卷 / 01期

基金：

美国国家卫生研究院;

关键词：

EMIT; enzyme multiplied immunoassay technique; hemagglutinin A; peptide detection; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; BETA-GALACTOSIDASE; LEUCONOSTOC-MESENTEROIDES; HOMOGENEOUS IMMUNOASSAYS; PROTEIN; SENSITIVITY; OPIATES; EMIT;

D O I：

10.1080/15321819.2011.538623

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A practical approach for constructing enzyme-multiplied immunoassay technique (EMIT)-based protein/ peptide assays is described. Normally used in small-molecule drug testing, EMIT is a homogeneous assay method that is attractive for its simplicity, sensitivity, and rapidity. The EMIT-based peptide/ protein assay was developed by conjugating a cysteine-modified HA peptide (from influenza hemagglutinin A) to the reporter enzyme, glucose-6-phosphate dehydrogenase. The 13-min assay gave a free HA limit of detection of 10 nM and proved effective for detection of a high-molecular-weight model protein tagged with HA. Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection.

引用

页码：1 / 17

页数：17

共 50 条

[1] ENZYME-MULTIPLIED IMMUNOASSAY
GALEN, RS
FORMAN, DW
OBRIEN, JE
BROWN, PA
LANCET, 1976, 2 (7990): : 853 - 853
[2] ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE IN ASSAY OF ANTIEPILEPTIC DRUGS IN VARIOUS SPECIES OF ANIMALS
SARHAN, N
BAGREL, A
ANNALES DE BIOLOGIE CLINIQUE, 1978, 36 (03) : 323 - 323
[3] EVALUATION OF ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE (EMIT) FOR DETERMINATION OF SERUM DIGOXIN
ROSENTHAL, AF
VARGAS, MG
KLASS, CS
CLINICAL CHEMISTRY, 1976, 22 (11) : 1899 - 1902
[4] MODIFIED ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE VALPROIC ACID TEST KIT
MEATHERALL, R
KRAHN, J
THERAPEUTIC DRUG MONITORING, 1995, 17 (01) : 104 - 105
[5] ADAPTATION OF THE ENZYME-MULTIPLIED IMMUNOASSAY FOR METHOTREXATE TO THE CENTRIFUGAL ANALYZER
FINLEY, PR
WILLIAMS, RJ
GRIFFITH, F
LICHTI, DA
CLINICAL CHEMISTRY, 1980, 26 (02) : 341 - 343
[6] COMPARISON OF ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE WITH FLUORESCENCE POLARIZATION IMMUNOASSAY FOR DETERMINATION OF GENTAMICIN AND TOBRAMYCIN LEVELS IN SERUM
POHLOD, DJ
SARAVOLATZ, LD
SOMERVILLE, MM
JOURNAL OF CLINICAL MICROBIOLOGY, 1984, 20 (05) : 866 - 868
[7] COMPARISON OF FLUORESCENT IMMUNOASSAY (FIA) AND ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE (EMIT) FOR MEASUREMENT OF SERUM CARBAMAZEPINE CONCENTRATION
CARL, GF
SMITH, DB
DUNN, LP
CLINICAL BIOCHEMISTRY, 1982, 15 (06) : 298 - 301
[8] Vancomycin serum concentrations in patients with renal dysfunction: A comparison of fluorescence polarization immunoassay and the enzyme-multiplied immunoassay technique
Peckman, HJ
Dupuis, RE
Sawyer, WT
Brouwer, KLR
Cross, RE
THERAPEUTIC DRUG MONITORING, 1996, 18 (06) : 647 - 653
[9] EXPERIENCES WITH THE ENZYME-MULTIPLIED IMMUNOASSAY CYCLOSPORINE SPECIFIC ASSAY IN A THERAPEUTIC DRUG-MONITORING LABORATORY
MORRIS, RG
SACCOIA, NC
SALLUSTIO, BC
FERGUSSON, LK
MANGAS, S
KASSAPIDIS, C
THERAPEUTIC DRUG MONITORING, 1993, 15 (05) : 410 - 413
[10] MORE SENSITIVE ENZYME-MULTIPLIED IMMUNOASSAY TECHNIQUE FOR PROCAINAMIDE AND N-ACETYLPROCAINAMIDE IN PLASMA, SERUM, AND URINE
HENRY, PR
DHRUV, RA
CLINICAL CHEMISTRY, 1988, 34 (05) : 957 - 960

← 1 2 3 4 5 →