Comparative analysis of microbial DNA extraction protocols for groundwater samples

被引：7
|
作者：
Purswani, Jessica [1 ]
Manuel Martin-Platero, Antonio [2 ]
Reboleiro-Rivas, Patricia [1 ]
Gonzalez-Lopez, Jesus [1 ,2 ]
Pozo, Clementina [1 ,2 ]
机构：
[1] Univ Granada, Inst Water Research, Environm Microbiol Grp, E-18071 Granada, Spain
[2] Univ Granada, Dept Microbiol, E-18071 Granada, Spain
来源：
ANALYTICAL BIOCHEMISTRY | 2011年 / 416卷 / 02期
关键词：
MSOP; Chelex; Epicentre; FastDNA; Fastprep; DNA extraction; TGGE; Groundwater samples; DIVERSITY;
D O I：
10.1016/j.ab.2011.05.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comparative analysis of four different DNA extraction protocols was performed to determine the best choice for groundwater microbial diversity studies using temperature gradient gel electrophoresis (TGGE) analysis. The methods used were a chelex-based method, a modified salting out procedure (MSOP), and the commercial kits Epicentre and FastDNA. Both commercial kits exhibited the greatest reproducibility in their methods; however, their band patterns were very different. The protocol that showed the highest diversity was the chelex-based method, and the one that showed the lowest diversity was the FastDNA kit. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：240 / 242
页数：3
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