Comparative analysis of microbial DNA extraction protocols for groundwater samples

被引：7
|
作者：
Purswani, Jessica [1 ]
Manuel Martin-Platero, Antonio [2 ]
Reboleiro-Rivas, Patricia [1 ]
Gonzalez-Lopez, Jesus [1 ,2 ]
Pozo, Clementina [1 ,2 ]
机构：
[1] Univ Granada, Inst Water Research, Environm Microbiol Grp, E-18071 Granada, Spain
[2] Univ Granada, Dept Microbiol, E-18071 Granada, Spain
来源：
ANALYTICAL BIOCHEMISTRY | 2011年 / 416卷 / 02期
关键词：
MSOP; Chelex; Epicentre; FastDNA; Fastprep; DNA extraction; TGGE; Groundwater samples; DIVERSITY;
D O I：
10.1016/j.ab.2011.05.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comparative analysis of four different DNA extraction protocols was performed to determine the best choice for groundwater microbial diversity studies using temperature gradient gel electrophoresis (TGGE) analysis. The methods used were a chelex-based method, a modified salting out procedure (MSOP), and the commercial kits Epicentre and FastDNA. Both commercial kits exhibited the greatest reproducibility in their methods; however, their band patterns were very different. The protocol that showed the highest diversity was the chelex-based method, and the one that showed the lowest diversity was the FastDNA kit. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：240 / 242
页数：3
相关论文
共 50 条
  • [31] Assessment of microbial DNA extraction methods of cadaver soil samples for criminal investigations
    Finley, Sheree J.
    Lorenco, Nathalie
    Mulle, Jennifer
    Robertson, Boakai K.
    Javan, Gulnaz T.
    AUSTRALIAN JOURNAL OF FORENSIC SCIENCES, 2016, 48 (03) : 265 - 272
  • [32] Comparative evaluation of different DNA extraction procedures from food samples
    Di Bernardo, G.
    Del Gaudio, S.
    Galderisi, U.
    Cascino, A.
    Cipollaro, M.
    BIOTECHNOLOGY PROGRESS, 2007, 23 (02) : 297 - 301
  • [33] Validation of a universal DNA extraction method for human and microbiAL DNA analysis
    Alessandrini, Federica
    Brenciani, Andrea
    Fioriti, Simona
    Melchionda, Filomena
    Mingoia, Marina
    Morroni, Gianluca
    Tagliabracci, Adriano
    FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2019, 7 (01) : 256 - 258
  • [34] Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
    de Almeida, Lauana Fogaca
    de Moraes, Leonardo Nazario
    dos Santos, Lucilene Delazari
    Valente, Guilherme Targino
    ANALYTICAL BIOCHEMISTRY, 2019, 567 : 90 - 95
  • [35] Genomic DNA extraction protocols for bone samples: The comparison of Qiagen and Zymo Research spin columns
    Vanek, Daniel
    Silerova, Marcela
    Urbanova, Vladislava
    Saskova, Lenka
    Dubska, Jitka
    Beran, Michal
    FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2011, 3 (01) : E397 - E398
  • [36] Evaluation and optimization of lysis method for microbial DNA extraction from epiphytic phyllosphere samples
    Yang, Seongmin
    Lee, Jaejin
    Mahmood, Maheen
    Min, Doohong
    Im, Jeongdae
    JOURNAL OF MICROBIOLOGICAL METHODS, 2023, 206
  • [37] Anionic nanocellulose as competing agent in microbial DNA extraction from mine process samples
    Bomberg, Malin
    Miettinen, Hanna
    JOURNAL OF MICROBIOLOGICAL METHODS, 2023, 215
  • [38] Commercial DNA extraction kits impact observed microbial community composition in permafrost samples
    Vishnivetskaya, Tatiana A.
    Layton, Alice C.
    Lau, Maggie C. Y.
    Chauhan, Archana
    Cheng, Karen R.
    Meyers, Arthur J.
    Murphy, Jasity R.
    Rogers, Alexandra W.
    Saarunya, Geetha S.
    Williams, Daniel E.
    Pfiffner, Susan M.
    Biggerstaff, John P.
    Stackhouse, Brandon T.
    Phelps, Tommy J.
    Whyte, Lyle
    Sayler, Gary S.
    Onstott, Tullis C.
    FEMS MICROBIOLOGY ECOLOGY, 2014, 87 (01) : 217 - 230
  • [39] Extracellular DNA in Environmental Samples: Occurrence, Extraction, Quantification, and Impact on Microbial Biodiversity Assessment
    Bairoliya, Sakcham
    Xiang, Jonas Koh Zhi
    Cao, Bin
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2022, 88 (03)
  • [40] Statistical assessment of DNA extraction methodology for culture-independent analysis of microbial community associated with diverse environmental samples
    Mahajan, Rishi
    Attri, Sampan
    Sharma, Kavita
    Singh, Niharika
    Sharma, Deepika
    Goel, Gunjan
    MOLECULAR BIOLOGY REPORTS, 2018, 45 (03) : 297 - 308
12345