A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

被引：69

作者：

Zhou, Rongxing ^{[1
]}

Li, Yongya ^{[2
,3
]}

Dong, Tianyu ^{[1
,2
,3
]}

Tang, Yanan ^{[2
]}

Li, Feng ^{[2
,3
]}

机构：

[1] Sichuan Univ, Biliary Surg Dept, West China Hosp, Chengdu, Sichuan, Peoples R China

[2] Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China

[3] Brock Univ, Dept Chem, 1812 Sir Isaac Brock Way, St Catharines, ON L3S 3A1, Canada

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 24期

关键词：

NUCLEIC-ACID AMPLIFICATION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; DNA; DIAGNOSTICS; CIRCUITS;

D O I：

10.1039/d0cc00397b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5 '-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.

引用

页码：3536 / 3538

页数：3

共 50 条

[41] CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage
Bao, Yijuan
Jiang, Yongzhong
Xiong, Erhu
Tian, Tian
Zhang, Zhenzhen
Lv, Jing
Li, Yang
Zhou, Xiaoming
ACS SENSORS, 2020, 5 (04) : 1082 - 1091
[42] An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus
Qiang Li
Chunyi Xue
Jianping Qin
Qingfeng Zhou
Feng Chen
Yingzuo Bi
Yongchang Cao
Archives of Virology, 2009, 154 : 1433 - 1440
[43] Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay
Liu, Chun-Yan
Song, Hui-Qun
Zhang, Ren-Li
Chen, Mu-Xin
Xu, Min-Jun
Ai, Lin
Chen, Xiao-Guang
Zhan, Xi-Mei
Liang, Shao-Hui
Yuan, Zi-Guo
Lin, Rui-Qing
Zhu, Xing-Quan
MOLECULAR AND CELLULAR PROBES, 2011, 25 (04) : 164 - 167
[44] Development and optimization of a loop-mediated isothermal amplification (LAMP) assay for the species-specific detection of Penicillium expansum
Frisch, Lisa M.
Mann, Magdalena A.
Marek, David N.
Niessen, Ludwig
FOOD MICROBIOLOGY, 2021, 95
[45] A Loop-Mediated Isothermal Amplification (LAMP) Assay Specific to Trichomonas tenax Is Suitable for Use at Point-of-Care
Matthew, Maurice A.
Christie, Jevan
Yang, Nawu
Yao, Chaoqun
MICROORGANISMS, 2022, 10 (03)
[46] A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis
Juscamayta-Lopez, Eduardo
Valdivia, Faviola
Soto, Maria Pia
Nurena, Brenda
Horna, Helen
SCIENTIFIC REPORTS, 2023, 13 (01)
[47] Rapid Detection of Hepatitis B Virus in Blood Plasma by a Specific and Sensitive Loop-Mediated Isothermal Amplification Assay
Nyan, Dougbeh-Chris
Ulitzky, Laura E.
Cehan, Nicoleta
Williamson, Phillip
Winkelman, Valerie
Rios, Maria
Taylor, Deborah R.
CLINICAL INFECTIOUS DISEASES, 2014, 59 (01) : 16 - 23
[48] Development of a novel and rapid loop-mediated isothermal amplification assay for specific detection of Alternaria alternata and Alternaria solani
Caiazzo, R.
Wood, T.
Thomas, J.
PHYTOPATHOLOGY, 2018, 108 (10) : 53 - 53
[49] A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis
Eduardo Juscamayta-López
Faviola Valdivia
María Pía Soto
Brenda Nureña
Helen Horna
Scientific Reports, 13
[50] CelB is a suitable marker for rapid and specific identification of Klebsiella pneumoniae by the loop-mediated isothermal amplification (LAMP) assay
Yichen Tian
Lefei Wang
Jinyang Zhang
Qinqin Han
Xue-shan Xia
Yuzhu Song
Guangying Yang
Brazilian Journal of Microbiology, 2019, 50 : 961 - 967

← 1 2 3 4 5 →