A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

被引：69

作者：

Zhou, Rongxing ^{[1
]}

Li, Yongya ^{[2
,3
]}

Dong, Tianyu ^{[1
,2
,3
]}

Tang, Yanan ^{[2
]}

Li, Feng ^{[2
,3
]}

机构：

[1] Sichuan Univ, Biliary Surg Dept, West China Hosp, Chengdu, Sichuan, Peoples R China

[2] Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China

[3] Brock Univ, Dept Chem, 1812 Sir Isaac Brock Way, St Catharines, ON L3S 3A1, Canada

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 24期

关键词：

NUCLEIC-ACID AMPLIFICATION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; DNA; DIAGNOSTICS; CIRCUITS;

D O I：

10.1039/d0cc00397b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5 '-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.

引用

页码：3536 / 3538

页数：3

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