A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a

被引：69
|
作者：
Zhou, Rongxing [1 ]
Li, Yongya [2 ,3 ]
Dong, Tianyu [1 ,2 ,3 ]
Tang, Yanan [2 ]
Li, Feng [2 ,3 ]
机构：
[1] Sichuan Univ, Biliary Surg Dept, West China Hosp, Chengdu, Sichuan, Peoples R China
[2] Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China
[3] Brock Univ, Dept Chem, 1812 Sir Isaac Brock Way, St Catharines, ON L3S 3A1, Canada
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 24期
关键词：
NUCLEIC-ACID AMPLIFICATION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; DNA; DIAGNOSTICS; CIRCUITS;
D O I：
10.1039/d0cc00397b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5 '-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.
引用
收藏
页码：3536 / 3538
页数：3
相关论文
共 50 条
  • [11] Rapid and Specific Colorimetric Assay for Detecting CRISPR-Cas9 in Genome-Edited Plants Using Loop-Mediated Isothermal Amplification
    Frango, Alberto
    Chen, Qiming
    Zhang, Hua
    Liu, Zhanmin
    FOOD ANALYTICAL METHODS, 2025,
  • [12] Quantitative analysis of DNA methylation using sequence-specific, real-time loop-mediated isothermal amplification
    Fu, Yao
    Zhang, Li
    Xing, Yu
    Deng, Shixiong
    ANALYTICA CHIMICA ACTA, 2022, 1235
  • [13] RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection of Phytophthora sojae
    Guo, Yufang
    Xia, Hongming
    Dai, Tingting
    Liu, Tingli
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2023, 13
  • [14] Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay
    Liu, Jiajia
    Tao, Dagang
    Chen, Xinquan
    Shen, Linyuan
    Zhu, Li
    Xu, Bingrong
    Liu, Hailong
    Zhao, Shuhong
    Li, Xinyun
    Liu, Xiangdong
    Xie, Shengsong
    Niu, Lili
    VIRUSES-BASEL, 2022, 14 (04):
  • [15] Development of a reverse transcription loop-mediated isothermal amplification based clustered regularly interspaced short palindromic repeats Cas12a assay for duck Tembusu virus
    Ding, Yangbao
    Huang, Zhanhong
    Li, Xinbo
    Tang, Mei
    Li, Weiqiang
    Feng, Siyu
    Zhao, Luxiang
    Zhang, Junsheng
    Yuan, Shichao
    Shan, Fen
    Jiao, Peirong
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [16] Efficient multiplexing and variant discrimination in reverse-transcription loop-mediated isothermal amplification with sequence-specific hybridization probes
    Zhang, Yinhua
    Tanner, Nathan A.
    BIOTECHNIQUES, 2022, 73 (05) : 247 - 255
  • [17] CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs
    Zhang, Mai
    Wang, Honghong
    Wang, Hui
    Wang, Fangfang
    Li, Zhengping
    ANALYTICAL CHEMISTRY, 2021, 93 (22) : 7942 - 7948
  • [18] Simple and specific detection of Bordetella holmesii by using a loop-mediated isothermal amplification assay
    Otsuka, Nao
    Yoshino, Shuji
    Kawano, Kimiko
    Toyoizumi-Ajisaka, Hiromi
    Shibayama, Keigo
    Kamachi, Kazunari
    MICROBIOLOGY AND IMMUNOLOGY, 2012, 56 (07) : 486 - 489
  • [19] Development of a loop-mediated isothermal amplification assay for rapid and specific detection of Xanthomonas perforans
    Dong, A.
    Hudson, O.
    Stackhouse, T. L.
    Waliullah, S.
    Ji, P.
    Ali, E.
    PHYTOPATHOLOGY, 2021, 111 (10) : 34 - 35
  • [20] Digital Recombinase Polymerase Amplification, Digital Loop-Mediated Isothermal Amplification, and Digital CRISPR-Cas Assisted Assay: Current Status, Challenges, and Perspectives
    Yin, Weihong
    Zhuang, Jianjian
    Li, Jiale
    Xia, Liping
    Hu, Kai
    Yin, Juxin
    Mu, Ying
    SMALL, 2023, 19 (49)
12345