Mechanical Characterization of a Single Yeast Cell Using a Robot Integrated Microfluidic Chip

被引：0
|
作者：
Chang, Di [1 ]
Sakuma, Shinya [1 ]
Uehara, Chihiro [2 ]
Uozumi, Nobuyuki [2 ]
Arai, Fumihito [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Dept Micronano Syst Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
来源：
2018 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS) | 2018年
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中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
This paper reports the evaluation results of Young's modulus of single Yeast cell (BY4741). We compared the Young's modulus of wild type and mutant type (Delta CCW12) using our constructed system. The system consists of a robot integrated microfluidic chip and a microscope with an optical tweezers system. The Young's modulus is measured by compressing the cell and measuring cellular reactive force. We succeeded in measuring wild type cells and mutant cells and compared their difference.
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