Mechanical Characterization of a Single Yeast Cell Using a Robot Integrated Microfluidic Chip

被引：0
|
作者：
Chang, Di [1 ]
Sakuma, Shinya [1 ]
Uehara, Chihiro [2 ]
Uozumi, Nobuyuki [2 ]
Arai, Fumihito [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Dept Micronano Syst Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
来源：
2018 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS) | 2018年
关键词：
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
This paper reports the evaluation results of Young's modulus of single Yeast cell (BY4741). We compared the Young's modulus of wild type and mutant type (Delta CCW12) using our constructed system. The system consists of a robot integrated microfluidic chip and a microscope with an optical tweezers system. The Young's modulus is measured by compressing the cell and measuring cellular reactive force. We succeeded in measuring wild type cells and mutant cells and compared their difference.
引用
收藏
页数：3
相关论文
共 50 条
  • [21] STIFFNESS-INDEX MAP BASED ON SINGLE CELL-SPHEROID ANALYSIS USING ROBOT INTEGRATED MICROFLUIDIC CHIIP
    Ito, Keitaro
    Sakuma, Shinya
    Kimura, Masaki
    Takebe, Takanori
    Kaneko, Makoto
    Arai, Fumihito
    2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS), 2016, : 157 - 160
  • [22] Integrated Biosensor with Microfluidic Chip and Microwave Sensor Chip for Cell Separation and Detection
    Li, Zhonghao
    Wang, Yanxiong
    Yang, Sen
    Chen, Qianlong
    Li, Yuanbo
    Liang, Junge
    Zhou, Xiaoman
    Fan, Qigao
    Jiang, Yanfeng
    Wang, Lei
    Qiang, Tian
    ADVANCED MATERIALS TECHNOLOGIES, 2025,
  • [23] An Integrated Microfluidic Chip and Its Clinical Application for Circulating Tumor Cell Isolation and Single-Cell Analysis
    Xu, Mingxin
    Zhao, Hui
    Chen, Jun
    Liu, Wenwen
    Li, Encheng
    Wang, Qi
    Zhang, Lichuan
    CYTOMETRY PART A, 2020, 97 (01) : 46 - 53
  • [24] An Evaluation Approach of Cell Viability Based on Cell Detachment Assay in a Single-Channel Integrated Microfluidic Chip
    Wei, Mingji
    Zhang, Rongbiao
    Zhang, Fei
    Zhang, Yecheng
    Li, Guoxiao
    Miao, Renjie
    Shao, Shihe
    ACS SENSORS, 2019, 4 (10) : 2654 - 2661
  • [25] Microfluidic Chip for Single-cell Interaction Anaysis
    Hsu, Chia-Hsien
    2019 13TH IEEE INTERNATIONAL CONFERENCE ON NANO/MOLECULAR MEDICINE & ENGINEERING (IEEE-NANOMED 2019), 2019, : 57 - 58
  • [26] Single-cell metabolite analysis on a microfluidic chip
    Wang, Chenlong
    Hu, Wanting
    Guan, Liandi
    Yang, Xiaoping
    Liang, Qionglin
    CHINESE CHEMICAL LETTERS, 2022, 33 (06) : 2883 - 2892
  • [27] Application of microfluidic chip systems for the research of single cell
    Gao, J
    Yin, XF
    Fang, ZL
    PROGRESS IN CHEMISTRY, 2004, 16 (06) : 975 - 983
  • [28] Single-cell metabolite analysis on a microfluidic chip
    Chenlong Wang
    Wanting Hu
    Liandi Guan
    Xiaoping Yang
    Qionglin Liang
    Chinese Chemical Letters, 2022, 33 (06) : 2883 - 2892
  • [29] Image processing and classification algorithm for yeast cell morphology in a microfluidic chip
    Yu, Bo Yang
    Elbuken, Caglar
    Ren, Carolyn L.
    Huissoon, Jan P.
    JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (06)
  • [30] LED induced fluorescence detector integrated in microfluidic cell chip
    Gu, Wenwen
    Huang, Jie
    Tan, Weimin
    INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, 2015, 12 (10-12) : 742 - 752
12345