Mechanical Characterization of a Single Yeast Cell Using a Robot Integrated Microfluidic Chip

被引:0
|
作者
Chang, Di [1 ]
Sakuma, Shinya [1 ]
Uehara, Chihiro [2 ]
Uozumi, Nobuyuki [2 ]
Arai, Fumihito [1 ]
机构
[1] Nagoya Univ, Grad Sch Engn, Dept Micronano Syst Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
关键词
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
This paper reports the evaluation results of Young's modulus of single Yeast cell (BY4741). We compared the Young's modulus of wild type and mutant type (Delta CCW12) using our constructed system. The system consists of a robot integrated microfluidic chip and a microscope with an optical tweezers system. The Young's modulus is measured by compressing the cell and measuring cellular reactive force. We succeeded in measuring wild type cells and mutant cells and compared their difference.
引用
收藏
页数:3
相关论文
共 50 条
  • [31] Automated measurement of cell mechanical properties using an integrated dielectrophoretic microfluidic device
    Yang, Hao
    Zhu, Mingjie
    Chen, Tao
    Niu, Fuzhou
    Sun, Lining
    Cheng, Liang
    ISCIENCE, 2022, 25 (05)
  • [32] Integrated Microfluidic Probe for Single Cell Manipulation
    Sofela, Samuel
    Saleh, Alla
    Qasaimeh, Mohammad A.
    PROCEEDINGS OF THE 5TH INTERNATIONAL CONFERENCE ON MANIPULATION, AUTOMATION, AND ROBOTICS AT SMALL SCALES (MARSS 2022), 2022,
  • [33] Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip
    Salzman, Valentina
    Torres, Moises R. Bustamante
    Tedesco, Francisco G. Correa
    Tarkowski, Nahuel
    Willems, Maria J. Godas
    Bravo, Joaquin N.
    Mercuri, Magali
    Mercado, Dante G.
    Berlin, Guido
    Bellino, Martin G.
    Aguilar, Pablo S.
    Estrada, Laura C.
    BIOLOGY OPEN, 2024, 13 (11):
  • [34] An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells
    Deng, Yuliang
    Zhang, Yu
    Sun, Shuai
    Wang, Zhihua
    Wang, Minjiao
    Yu, Beiqin
    Czajkowsky, Daniel M.
    Liu, Bingya
    Li, Yan
    Wei, Wei
    Shi, Qihui
    SCIENTIFIC REPORTS, 2014, 4
  • [35] An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells
    Yuliang Deng
    Yu Zhang
    Shuai Sun
    Zhihua Wang
    Minjiao Wang
    Beiqin Yu
    Daniel M. Czajkowsky
    Bingya Liu
    Yan Li
    Wei Wei
    Qihui Shi
    Scientific Reports, 4
  • [36] Integrated microfluidic systems for cell culture and characterization
    Flueckiger, Jonas
    Cheung, Karen C.
    2008 IEEE 14TH INTERNATIONAL MIXED-SIGNALS, SENSORS, AND SYSTEMS TEST WORKSHOP, 2008, : 154 - 159
  • [37] Single-Cell Enzyme-Free Dissociation of Neurospheres Using a Microfluidic Chip
    Lin, Ching-Hui
    Lee, Don-Ching
    Chang, Hao-Chen
    Chiu, Ing-Ming
    Hsu, Chia-Hsien
    ANALYTICAL CHEMISTRY, 2013, 85 (24) : 11920 - 11928
  • [38] Single Cell Detection Using Self-mixing Interferometry Based On Microfluidic Chip
    Zhang, Menglei
    Zhao, Yu
    Li, Jiawei
    Chen, Tao
    AOPC 2021: BIOMEDICAL OPTICS, 2021, 12067
  • [39] High-Throughput Single Cell Trapping and Patterning Using a Sandwiched Microfluidic Chip
    Fan, Lei
    Luo, Tao
    Sun, Dong
    2018 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND BIOMIMETICS (ROBIO), 2018, : 1508 - 1513
  • [40] MICROFLUIDIC CHIP FOR SIMULTANEOUS LOADING AND INJECTION OF A SINGLE CELL BY USING RACK AND PINION MECHANISMS
    Uning, Kevin T.
    Ichikawa, Keita
    Hirao, Akiho
    Michimoto, Taiga
    Sato, Tasuku
    Kume, Hiroaki
    2020 33RD IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2020), 2020, : 392 - 395