Amperometric Tyrosinase Biosensor Based on Carbon Nanotube-Doped Sol-Gel-Derived Zinc Oxide-Nafion Composite Films

被引：27
|
作者：
Lee, Joong Min [1 ,2 ]
Xu, Guang-Ri [3 ]
Kim, Byung Kun [1 ,2 ]
Choi, Han Nim [1 ,2 ]
Lee, Won-Yong [1 ,2 ]
机构：
[1] Yonsei Univ, Dept Chem, Seoul 120749, South Korea
[2] Yonsei Univ, Ctr Bioact Mol Hybrids, Seoul 120749, South Korea
[3] Henan Inst Sci & Technol, Xinxiang 453003, Peoples R China
来源：
ELECTROANALYSIS | 2011年 / 23卷 / 04期
关键词：
Biosensor; Phenol; Tyrosinase; Carbon nanotube; ZnO-Nafion composite; PHENOLIC-COMPOUNDS; MATRIX; SENSOR; CHEMILUMINESCENCE; WATER;
D O I：
10.1002/elan.201000556
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17 nm) of the composite film, the biosensor exhibits very fast response time of 2 s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7 x 10(-8) M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.
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页码：962 / 970
页数：9
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