Amperometric Tyrosinase Biosensor Based on Carbon Nanotube-Doped Sol-Gel-Derived Zinc Oxide-Nafion Composite Films

被引：27
|
作者：
Lee, Joong Min [1 ,2 ]
Xu, Guang-Ri [3 ]
Kim, Byung Kun [1 ,2 ]
Choi, Han Nim [1 ,2 ]
Lee, Won-Yong [1 ,2 ]
机构：
[1] Yonsei Univ, Dept Chem, Seoul 120749, South Korea
[2] Yonsei Univ, Ctr Bioact Mol Hybrids, Seoul 120749, South Korea
[3] Henan Inst Sci & Technol, Xinxiang 453003, Peoples R China
来源：
ELECTROANALYSIS | 2011年 / 23卷 / 04期
关键词：
Biosensor; Phenol; Tyrosinase; Carbon nanotube; ZnO-Nafion composite; PHENOLIC-COMPOUNDS; MATRIX; SENSOR; CHEMILUMINESCENCE; WATER;
D O I：
10.1002/elan.201000556
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17 nm) of the composite film, the biosensor exhibits very fast response time of 2 s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7 x 10(-8) M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.
引用
收藏
页码：962 / 970
页数：9
相关论文
共 50 条
  • [21] Investigation of aluminum content on the properties of Sol-Gel-derived zinc oxide thin films
    Xue, S. W.
    Zu, X. T.
    PRICM 6: SIXTH PACIFIC RIM INTERNATIONAL CONFERENCE ON ADVANCED MATERIALS AND PROCESSING, PTS 1-3, 2007, 561-565 : 1173 - 1176
  • [22] Sol-gel-derived amperometric glucose biosensor based on covalent attachment of toluidine blue O to carrier
    Qian, JM
    Li, XX
    Huang, HY
    POLYMERS FOR ADVANCED TECHNOLOGIES, 2003, 14 (3-5) : 207 - 211
  • [23] Hydrogen peroxide biosensor based on sol-gel-derived glasses doped with Eastman AQ polymer
    Chen, X
    Zhang, JZ
    Wang, BQ
    Cheng, GJ
    Dong, SJ
    ANALYTICA CHIMICA ACTA, 2001, 434 (02) : 255 - 260
  • [24] Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor
    Wang, B
    Zhang, J
    Dong, S
    BIOSENSORS & BIOELECTRONICS, 2000, 15 (7-8): : 397 - 402
  • [25] Sol-gel-derived cobalt phthalocyanine-dispersed carbon composite electrodes for electrocatalysis and amperometric flow detection
    Wang, J
    Pamidi, PVA
    Parrado, C
    Park, DS
    Pingarron, J
    ELECTROANALYSIS, 1997, 9 (12) : 908 - 911
  • [26] Sol-Gel-Derived Cobalt Phthalocyanine-Dispersed Carbon Composite Electrodes for Electrocatalysis and Amperometric Flow Detection
    Electroanalysis, 9 (12):
  • [27] Sol-gel-derived nanocrystalline aluminum-doped zinc oxide thin films for use as antireflection coatings in silicon solar cells
    Verma, Amita
    Vijayan, Narayanasamy
    JOURNAL OF MATERIALS RESEARCH, 2013, 28 (21) : 2990 - 2995
  • [28] Effects of individual layer thickness on the microstructure and optoelectronic properties of sol-gel-derived zinc oxide thin films
    Tahar, Noureddine Bel Hadj
    Tahar, Radhouane Bel Hadj
    Ben Salah, Abdelhamid
    Savall, Andre
    JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 2008, 91 (03) : 846 - 851
  • [29] Renewable silica sol-gel derived carbon composite based glucose biosensor
    Li, J
    Chia, LS
    Goh, NK
    Tan, SN
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 460 (1-2): : 234 - 241
  • [30] Green cathodoluminescenee properties of zinc oxide films prepared by excimer laser irradiation of a sol-gel-derived precursor
    Nagase, T
    Ooie, T
    Kominami, H
    Nakanishi, Y
    Mizutani, N
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 2003, 42 (03): : 1179 - 1184
12345