Amperometric Tyrosinase Biosensor Based on Carbon Nanotube-Doped Sol-Gel-Derived Zinc Oxide-Nafion Composite Films

被引：27

作者：

Lee, Joong Min ^{[1
,2
]}

Xu, Guang-Ri ^{[3
]}

Kim, Byung Kun ^{[1
,2
]}

Choi, Han Nim ^{[1
,2
]}

Lee, Won-Yong ^{[1
,2
]}

机构：

[1] Yonsei Univ, Dept Chem, Seoul 120749, South Korea

[2] Yonsei Univ, Ctr Bioact Mol Hybrids, Seoul 120749, South Korea

[3] Henan Inst Sci & Technol, Xinxiang 453003, Peoples R China

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 04期

关键词：

Biosensor; Phenol; Tyrosinase; Carbon nanotube; ZnO-Nafion composite; PHENOLIC-COMPOUNDS; MATRIX; SENSOR; CHEMILUMINESCENCE; WATER;

D O I：

10.1002/elan.201000556

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17 nm) of the composite film, the biosensor exhibits very fast response time of 2 s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7 x 10(-8) M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.

引用

页码：962 / 970

页数：9

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