Flow injection amperometric detection of 2′-deoxyguanosine at a ruthenium oxide hexacyanoferrate modified electrode

A ruthenium oxide hexacyanoferrate (RuOHCF) modified electrode was developed. Hydrodynamic voltammetry was employed to demonstrate the remarkable electrocatalytic activity toward the oxidation of 2 '-deoxyguanosine. The RuOHCF modified electrode was used as amperometric detector for 2 '-deoxyguanosine determination in a FIA apparatus. The influence of various experimental conditions was explored for optimum analytical performance, and at these experimental conditions, the method exhibited a linear response range to 2 '-deoxyguanosine extending from 3.8 to 252 mu mol L-1 with detection limit of 94 nmol L-1. Applications in DNA samples were examined, and the results for determination of 2 '-deoxyguanosine were in good agreement with those obtained by HPLC analysis. Studies on the kinetics of the in vitro consumption of 2 '-deoxyguanosine by acetaldehyde were also performed.