PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein

被引：0
|
作者：
Gumusel, F
Kiremit, N
Issever, S
Ogras, TT
Bermek, E
机构：
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1996年 / 38卷 / 02期
关键词：
penicillin acylase; pac; PCR; cloning; expression;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.
引用
收藏
页码：315 / 324
页数：10
相关论文
共 50 条
  • [41] Cloning of an alpha-glucosidase gene from Arabidopsis and expression in E-coli of a portion of the coding sequence.
    Hall, BD
    Wright, PW
    Monroe, JD
    PLANT PHYSIOLOGY, 1996, 111 (02) : 536 - 536
  • [42] Soluble expression and partial purification of recombinant human erythropoietin from E-coli
    Jeong, Taeck-Hyun
    Son, Young-Jin
    Ryu, Han-Bong
    Koo, Bon-Kyung
    Jeong, Seung-Mi
    Hoang, Phuong
    Do, Bich Hang
    Song, Jung-A
    Chong, Seon-Ha
    Robinson, Robert Charles
    Choe, Han
    PROTEIN EXPRESSION AND PURIFICATION, 2014, 95 : 211 - 218
  • [43] Preparation of functional recombinant protein from E-coli using a nondetergent sulfobetaine
    Chong, YC
    Chen, HW
    BIOTECHNIQUES, 2000, 29 (06) : 1166 - 1167
  • [44] Molecular cloning of a cDNA encoding ribosome inactivating protein from Amaranthus viridis and its expression in E-coli
    Kwon, SY
    An, CS
    Liu, JR
    Kwak, SS
    Lee, HS
    Kim, JK
    Paek, KH
    MOLECULES AND CELLS, 2000, 10 (01) : 8 - 12
  • [45] Cloning and soluble expression of mature α-luffin from Luffa cylindrica in E-coli using SUMO fusion protein
    Namvar, Shaghayegh
    Barkhordari, Farzaneh
    Raigani, Mozhgan
    Jahandar, Hoda
    Nematollahi, Leila
    Davami, Fatemeh
    TURKISH JOURNAL OF BIOLOGY, 2018, 42 (01) : 23 - 32
  • [46] Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
    Ajamaluddin Malik
    3 Biotech, 2016, 6
  • [47] Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
    Malik, Ajamaluddin
    3 BIOTECH, 2016, 6 : 1 - 7
  • [48] Purification and Characterization of a recombinant β-Xylosidase from Bacillus licheniformis ATCC 14580 into E-coli B121
    Zafar, Asma
    Aftab, Muhammad Nauman
    Manzoor, Asma
    Iqbal, Irfana
    Din, Zia Ud
    Kaleem, Afshan
    Khooharo, Ateeque Rahman
    Saleem, Mushtaq Ahmad
    PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2018, 31 (06) : 2755 - 2762
  • [49] Cloning and expression of a pepstatin insensitive acid protease from Thermoplasma volcanium in E-coli
    Koyuncu, B
    Ozel, H
    Kocabiyik, S
    FEBS JOURNAL, 2005, 272 : 156 - 156
  • [50] Expression of the soluble alternatively spliced erythropoietin receptor in E-coli and purification of the recombinant protein.
    Baynes, RD
    Wu, W
    Baynes, TL
    Flanigan, T
    Padmanabhan, R
    BLOOD, 1996, 88 (10) : 3253 - 3253
12345