PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein

被引：0
|
作者：
Gumusel, F
Kiremit, N
Issever, S
Ogras, TT
Bermek, E
机构：
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1996年 / 38卷 / 02期
关键词：
penicillin acylase; pac; PCR; cloning; expression;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.
引用
收藏
页码：315 / 324
页数：10
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