PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein

被引：0
|
作者：
Gumusel, F
Kiremit, N
Issever, S
Ogras, TT
Bermek, E
机构：
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1996年 / 38卷 / 02期
关键词：
penicillin acylase; pac; PCR; cloning; expression;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.
引用
收藏
页码：315 / 324
页数：10
相关论文
共 50 条
  • [21] Simplified purification process for the recovery of a soluble recombinant protein expressed in E-coli periplasmic space.
    Hsieh, JH
    Spence, C
    Corrigan, R
    Porter, J
    Bailon, P
    Berthold, W
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1998, 216 : U269 - U269
  • [22] Cloning and expression of the streptavidin gene from Streptomyces avidinii in Escherichia coli and secretion of streptavidin by E-coli cells
    Veiko, VP
    Gul'ko, LB
    Okorokova, NA
    D'yakov, NA
    Debabov, VG
    BIOORGANICHESKAYA KHIMIYA, 1999, 25 (03): : 184 - 188
  • [23] Cloning, sequencing and expression of a swine IgG H chain gene in E-coli
    Zhao, K
    Zheng, ZX
    CHINESE SCIENCE BULLETIN, 1999, 44 (09): : 821 - 825
  • [24] Cloning and expression in E-coli of Chlamydomonas reinhardtii CrFtsZ3 gene
    Hu, Y
    Kong, DD
    Wang, D
    He, YK
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2003, 35 (11): : 998 - 1004
  • [25] Tunable recombinant protein expression with E-coli in a mixed-feed environment
    Sagmeister, Patrick
    Schimek, Clemens
    Meitz, Andrea
    Herwig, Christoph
    Spadiut, Oliver
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (07) : 2937 - 2945
  • [26] Expression, Purification and Identification of Recombinant Mouse Interleukin 21 Protein in E-coli
    Tang, Weiguo
    Chen, Guobing
    Gu, Qing
    Pan, Jing
    Wu, Wutong
    CELLULAR & MOLECULAR IMMUNOLOGY, 2006, 3 (04) : 311 - 315
  • [27] Genetic selection system for improving recombinant membrane protein expression in E-coli
    Massey-Gendel, Elizabeth
    Zhao, Anni
    Boulting, Gabriella
    Kim, Hye-Yeon
    Balamotis, Michael A.
    Seligman, Len M.
    Nakamoto, Robert K.
    Bowie, James U.
    PROTEIN SCIENCE, 2009, 18 (02) : 372 - 383
  • [28] Cloning, E-coli expression and molecular analysis of a novel sesquiterpene synthase gene from Artemisia annua
    Liu, Y
    Ye, HC
    Li, GF
    ACTA BOTANICA SINICA, 2002, 44 (12): : 1450 - 1455
  • [29] Cloning and expression in E-coli of the HOPDA hydrolase gene from Pseudomonas sp P20
    Lim, JC
    Chae, JC
    Kim, YS
    Kim, HB
    Kim, CK
    JOURNAL OF MICROBIOLOGY, 1996, 34 (04) : 349 - 354
  • [30] Cloning and expression of L-asparaginase from E-coli in eukaryotic expression system
    Sajitha, Syed
    Vidya, Jalaja
    Varsha, Karunakaran
    Binod, Parameswaran
    Pandey, Ashok
    BIOCHEMICAL ENGINEERING JOURNAL, 2015, 102 : 14 - 17
12345