Urine protein concentration estimation for biomarker discovery

被引：6

作者：

Mistry, Hiten D. ^{[1
]}

Bramham, Kate ^{[1
]}

Weston, Andrew J. ^{[2
]}

Ward, Malcolm A. ^{[2
]}

Thompson, Andrew J. ^{[2
,3
]}

Chappell, Lucy C. ^{[1
]}

机构：

[1] Kings Coll London, Div Womens Hlth, London SE1 7EH, England

[2] Kings Coll London, Inst Psychiat, Prote Facil, Ctr Excellence Mass Spectrometry, London SE1 7EH, England

[3] Inst Canc Res, Prote Core Facil, London SW3 6JB, England

来源：

PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH | 2013年 / 3卷 / 04期

关键词：

Protein concentration assays; Proteomics; Urine; PROTEOMICS; PREGNANCY;

D O I：

10.1016/j.preghy.2013.07.001

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the 'gold standard' amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation. (C) 2013 International Society for the Study of Hypertension in Pregnancy Published by Elsevier B.V. All rights reserved.

引用

页码：211 / 214

页数：4

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