Urine protein concentration estimation for biomarker discovery

被引：6
|
作者：
Mistry, Hiten D. [1 ]
Bramham, Kate [1 ]
Weston, Andrew J. [2 ]
Ward, Malcolm A. [2 ]
Thompson, Andrew J. [2 ,3 ]
Chappell, Lucy C. [1 ]
机构：
[1] Kings Coll London, Div Womens Hlth, London SE1 7EH, England
[2] Kings Coll London, Inst Psychiat, Prote Facil, Ctr Excellence Mass Spectrometry, London SE1 7EH, England
[3] Inst Canc Res, Prote Core Facil, London SW3 6JB, England
来源：
PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH | 2013年 / 3卷 / 04期
关键词：
Protein concentration assays; Proteomics; Urine; PROTEOMICS; PREGNANCY;
D O I：
10.1016/j.preghy.2013.07.001
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the 'gold standard' amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation. (C) 2013 International Society for the Study of Hypertension in Pregnancy Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：211 / 214
页数：4
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