Integrated and automated high-throughput purification of libraries on microscale

被引：9

作者：

Ginsburg-Moraff, Carol ^{[2
]}

Grob, Jonathan ^{[2
,4
]}

Chin, Karl ^{[2
]}

Eastman, Grant ^{[2
]}

Wildhaber, Sandra ^{[1
]}

Bayliss, Mark ^{[3
]}

Mues, Heinrich M. ^{[1
]}

Palmieri, Marco ^{[1
]}

Poirier, Jennifer

Reck, Marcel ^{[1
]}

Luneau, Alexandre ^{[1
]}

Rodde, Stephane ^{[1
]}

Reilly, John ^{[1
]}

Wagner, Trixie ^{[1
]}

Brocklehurst, Cara E. ^{[1
]}

Wyler, Rene ^{[1
]}

Dunstan, David ^{[2
,5
]}

Marziale, Alexander N. ^{[1
,6
]}

机构：

[1] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Fabrikstr 1, CH-4056 Basel, Switzerland

[2] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Cambridge, MA 02139 USA

[3] Virscidian Inc, Cary, NC 27511 USA

[4] Valo Hlth, 75 Hayden Ave, Lexington, MA 02421 USA

[5] Relay Therapeut, 399 Binney St, Cambridge, MA 02139 USA

[6] AstraZeneca, Pepparedsleden 1, S-43183 Molndal, Sweden

来源：

SLAS TECHNOLOGY | 2022年 / 27卷 / 06期

关键词：

High-throughput purification; Laboratory automation; Library purification; Microscale libraries; Analytical chemistry automation; PERFORMANCE LIQUID-CHROMATOGRAPHY; CHARGED AEROSOL DETECTION; UNIVERSAL DETECTORS; CHEMISTRY; GENERATION; INHIBITORS; PLATFORM; IDENTIFICATION; IMPURITIES; MODULATORS;

D O I：

10.1016/j.slast.2022.08.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We herein report the development of an automation platform for rapid purification and quantification of chem-ical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on ana-lytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.

引用

页码：350 / 360

页数：11

共 50 条

[21] High-throughput screening of catalyst libraries
不详
CHEMICAL & ENGINEERING NEWS, 1998, 76 (30) : 49 - 49
[22] High-throughput screening of enzyme libraries
Olsen, M
Iverson, B
Georgiou, G
CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (04) : 331 - 337
[23] Automated high-throughput Wannierisation
Vitale, Valerio
Pizzi, Giovanni
Marrazzo, Antimo
Yates, Jonathan R.
Marzari, Nicola
Mostofi, Arash A.
NPJ COMPUTATIONAL MATERIALS, 2020, 6 (01)
[24] Parallel purification of microscale libraries via automated solid phase extraction
Wleklinski, Michael
Carpenter, Paige M.
Dykstra, Kevin D.
Donofrio, Anthony
Nowak, Timothy
Krska, Shane W.
Ferguson, Ronald D.
SLAS TECHNOLOGY, 2024, 29 (02): : 100126
[25] Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system
Baier, J
Kaufman, J
Mason, G
Wang, J
Wright, P
Andrus, A
BIOTECHNIQUES, 1996, 20 (02) : 298 - 303
[26] rAAV Production and Titration at the Microscale for High-Throughput Screening
Quan, David Nathan
Shiloach, Joseph
HUMAN GENE THERAPY, 2022, 33 (1-2) : 94 - 102
[27] High-throughput purification of combinatorial arrays
Edwards, C
Hunter, DJ
JOURNAL OF COMBINATORIAL CHEMISTRY, 2003, 5 (01): : 61 - 66
[28] High-throughput evaluation of the wettability of polymer libraries
Thaburet, JFO
Mizomoto, H
Bradley, M
MACROMOLECULAR RAPID COMMUNICATIONS, 2004, 25 (01) : 366 - 370
[29] Generation of RNAi libraries for high-throughput screens
Clark, Julie
Ding, Sheng
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2006,
[30] Automated equipment for high-throughput experimentation
Brändli, C
Maiwald, P
Schröer, J
CHIMIA, 2003, 57 (05) : 284 - 289

← 1 2 3 4 5 →