Integrated and automated high-throughput purification of libraries on microscale

被引：9

作者：

Ginsburg-Moraff, Carol ^{[2
]}

Grob, Jonathan ^{[2
,4
]}

Chin, Karl ^{[2
]}

Eastman, Grant ^{[2
]}

Wildhaber, Sandra ^{[1
]}

Bayliss, Mark ^{[3
]}

Mues, Heinrich M. ^{[1
]}

Palmieri, Marco ^{[1
]}

Poirier, Jennifer

Reck, Marcel ^{[1
]}

Luneau, Alexandre ^{[1
]}

Rodde, Stephane ^{[1
]}

Reilly, John ^{[1
]}

Wagner, Trixie ^{[1
]}

Brocklehurst, Cara E. ^{[1
]}

Wyler, Rene ^{[1
]}

Dunstan, David ^{[2
,5
]}

Marziale, Alexander N. ^{[1
,6
]}

机构：

[1] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Fabrikstr 1, CH-4056 Basel, Switzerland

[2] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Cambridge, MA 02139 USA

[3] Virscidian Inc, Cary, NC 27511 USA

[4] Valo Hlth, 75 Hayden Ave, Lexington, MA 02421 USA

[5] Relay Therapeut, 399 Binney St, Cambridge, MA 02139 USA

[6] AstraZeneca, Pepparedsleden 1, S-43183 Molndal, Sweden

来源：

SLAS TECHNOLOGY | 2022年 / 27卷 / 06期

关键词：

High-throughput purification; Laboratory automation; Library purification; Microscale libraries; Analytical chemistry automation; PERFORMANCE LIQUID-CHROMATOGRAPHY; CHARGED AEROSOL DETECTION; UNIVERSAL DETECTORS; CHEMISTRY; GENERATION; INHIBITORS; PLATFORM; IDENTIFICATION; IMPURITIES; MODULATORS;

D O I：

10.1016/j.slast.2022.08.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We herein report the development of an automation platform for rapid purification and quantification of chem-ical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on ana-lytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.

引用

页码：350 / 360

页数：11

共 50 条

[41] Preparative high-performance liquid chromatography-mass spectrometry for the high-throughput purification of combinatorial libraries
Bauser, M
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2002, 40 (05) : 292 - 296
[42] An integrated platform for high-throughput nanoscopy
Barentine, Andrew E. S.
Lin, Yu
Courvan, Edward M.
Kidd, Phylicia
Liu, Miao
Balduf, Leonhard
Phan, Timy
Rivera-Molina, Felix
Grace, Michael R.
Marin, Zach
Lessard, Mark
Chen, Juliana Rios
Wang, Siyuan
Neugebauer, Karla M.
Bewersdorf, Joerg
Baddeley, David
NATURE BIOTECHNOLOGY, 2023, 41 (11) : 1549 - +
[43] An integrated platform for high-throughput nanoscopy
Andrew E. S. Barentine
Yu Lin
Edward M. Courvan
Phylicia Kidd
Miao Liu
Leonhard Balduf
Timy Phan
Felix Rivera-Molina
Michael R. Grace
Zach Marin
Mark Lessard
Juliana Rios Chen
Siyuan Wang
Karla M. Neugebauer
Joerg Bewersdorf
David Baddeley
Nature Biotechnology, 2023, 41 : 1549 - 1556
[44] High-throughput plasmid purification for capillary sequencing
Elkin, CJ
Richardson, PM
Fourcade, HM
Hammon, NM
Pollard, MJ
Predki, PF
Glavina, T
Hawkins, TL
GENOME RESEARCH, 2001, 11 (07) : 1269 - 1274
[45] High-throughput expression and purification of membrane proteins
Mancia, Filippo
Love, James
JOURNAL OF STRUCTURAL BIOLOGY, 2010, 172 (01) : 85 - 93
[46] Techniques for analysis and purification in high-throughput chemistry
Hughes, I
Hunter, D
CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (03) : 243 - 247
[47] An integrated targeted metabolomic platform for high-throughput metabolite profiling and automated data processing
Yuping Cai
Kai Weng
Yuan Guo
Jie Peng
Zheng-Jiang Zhu
Metabolomics, 2015, 11 : 1575 - 1586
[48] An integrated targeted metabolomic platform for high-throughput metabolite profiling and automated data processing
Cai, Yuping
Weng, Kai
Guo, Yuan
Peng, Jie
Zhu, Zheng-Jiang
METABOLOMICS, 2015, 11 (06) : 1575 - 1586
[49] Acidity Characterization of Catalyst Libraries by High-Throughput Testing
G. Morra
D. Farrusseng
E. Guillon
S. Morin
C. Bouchy
C. Mirodatos
Topics in Catalysis, 2010, 53 : 49 - 56
[50] High-throughput testing of heterogeneous catalyst libraries.
Senkan, SM
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1999, 218 : U171 - U171

← 1 2 3 4 5 →