Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen

被引：0
|
作者：
JIN
机构：
来源：
Chinese Journal of Chemistry | 1994年 / 04期
关键词：
Biosensor; cholesterol oxidase; enzyme electrode;
D O I：
暂无
中图分类号：
O657.1 [电化学分析法];
学科分类号：
摘要：
Cholesterol oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize cholesterol oxidase and cholesterol esterase on a glassy carbon electrode modified with Nafion and methyl viologen. The biosensor has been used to determine total cholesterol in blood. The linear range of the determination is 2.5×10to 1.0×10-4 mol/L. The detection limit is about 5.0×10mol/L. The response time is 12 s. This biosensor has the advantage of high selectivity, sensitivity and short response time.
引用
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页码：343 / 347
页数：5
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