Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen

被引：0
|
作者：
JIN
机构：
来源：
Chinese Journal of Chemistry | 1994年 / 04期
关键词：
Biosensor; cholesterol oxidase; enzyme electrode;
D O I：
暂无
中图分类号：
O657.1 [电化学分析法];
学科分类号：
摘要：
Cholesterol oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize cholesterol oxidase and cholesterol esterase on a glassy carbon electrode modified with Nafion and methyl viologen. The biosensor has been used to determine total cholesterol in blood. The linear range of the determination is 2.5×10to 1.0×10-4 mol/L. The detection limit is about 5.0×10mol/L. The response time is 12 s. This biosensor has the advantage of high selectivity, sensitivity and short response time.
引用
收藏
页码：343 / 347
页数：5
相关论文
共 50 条
  • [1] Cholesterol oxidase biosensor based on a glassy carbon electrode modified with nafion and methyl viologen
    Jin, Li-Tong
    Zhao, Gui-Zhu
    Fang, }u-Zhi
    Chinese Journal of Chemistry (English Edition), 1994, 12 (04):
  • [2] A STUDY OF URICASE BIOSENSOR BASED ON A GLASSY-CARBON ELECTRODE MODIFIED WITH NAFION AND METHYL VIOLOGEN
    JIN, LT
    YE, JS
    TONG, W
    FANG, YZ
    MIKROCHIMICA ACTA, 1993, 112 (1-4) : 71 - 75
  • [3] Amperometric and impedimetric characterization of a glutamate biosensor based on Nafion® and a methyl viologen modified glassy carbon electrode
    Maalouf, Rita
    Chebib, Hanna
    Saikali, Youssef
    Vittori, Olivier
    Sigaud, Monique
    Jaffrezic-Renault, Nicole
    BIOSENSORS & BIOELECTRONICS, 2007, 22 (11): : 2682 - 2688
  • [4] Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode
    Haritha, V. S.
    Kumar, S. R. Sarath
    Rakhi, R. B.
    MATERIALS TODAY-PROCEEDINGS, 2022, 50 : 34 - 39
  • [5] Electrochemiluminescence Biosensor for Glucose Based on Graphene/Nafion/GOD Film Modified Glassy Carbon Electrode
    Chen, Xiaoping
    Ye, Huazhen
    Wang, Wanzong
    Qui, Bin
    Lin, Zhenyu
    Chen, Guonan
    ELECTROANALYSIS, 2010, 22 (20) : 2347 - 2352
  • [6] Amperometric cholesterol biosensor based on the direct electrochemistry of cholesterol oxidase and catalase on a graphene/ionic liquid-modified glassy carbon electrode
    Gholivand, Mohammad Bagher
    Khodadadian, Mehdi
    BIOSENSORS & BIOELECTRONICS, 2014, 53 : 472 - 478
  • [7] A Nitrite Biosensor Based on Coimmobilization of Nitrite Reductase and Viologen-Modified Polysiloxane on Glassy Carbon Electrode
    Quan, De
    Nagarale, R. K.
    Shin, Woonsup
    ELECTROANALYSIS, 2010, 22 (20) : 2389 - 2398
  • [8] Electrochemical Biosensor of Glucose Based on the Immobilization of Glucose Oxidase on a Carbon Microsphere Modified Glassy Carbon Electrode
    Dong, Yong Ping
    Huang, Li
    Lei, Zhi Ping
    Chu, Xiang Feng
    Zhang, Qian Feng
    SENSOR LETTERS, 2012, 10 (3-4) : 1024 - 1030
  • [9] Electrochemical characterization of biosensor based on nitrite reductase and methyl viologen co-immobilized glassy carbon electrode
    Quan, De
    Min, Dong Geun
    Cha, Geun Sig
    Nam, Hakhyun
    BIOELECTROCHEMISTRY, 2006, 69 (02) : 267 - 275
  • [10] Acetylcholinesterase Biosensor for the Detection of Methyl Parathion at an Electrochemically Reduced Graphene Oxide-Nafion Modified Glassy Carbon Electrode
    Jeyapragasam, Tharini
    Saraswathi, R.
    Chen, Shen-Ming
    Chen, Tse-Wei
    INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2017, 12 (06): : 4768 - 4781
12345