Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction

被引：0
|
作者：
Slyadnev, M.N. [1 ,2 ]
Lavrova, M.V. [1 ,2 ]
Erkin, M.A. [1 ]
Kazakov, V.A. [2 ]
Ganeev, A.A. [1 ]
机构：
[1] Department of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets 198504, Russia
[2] NPF AP Lumex, Moskovskii pr. 19, St. Petersburg 190005, Russia
来源：
Journal of Analytical Chemistry | 2008年 / 63卷 / 02期
关键词：
A microfluidic system that allowed us to perform the real-time polymerase chain reaction (PCR) in a glass-silicon microchip containing nine 250-nL microreactors was developed and studied. The resulting high heating/cooling rates of a PCR mixture in a microreactor allowed us to optimize the amplification mode (1 min/cycle). The silicon surface of microreactors was successfully passivated. The resulting analytical system allowed us to measure the PCR kinetic curves in chip microreactors at a DNA concentration of ∼5 × 104 copies per microreactor. It was found that; if the PCR is performed in a microchip with real-time detection using the optimized amplification mode; the result can be obtained 13-14 min after the onset of reaction. © Pleiades Publishing; Ltd; 2008;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：192 / 198
相关论文
共 50 条
  • [41] Development and Comparison of Real-Time Polymerase Chain Reaction and Real-Time Recombinase Polymerase Amplification Assays for Detection of Clostridium perfringens in Food
    Liu, Libing
    Li, Ruiwen
    Chen, Zhimin
    Wang, Jinfeng
    Sun, Xiaoxia
    Yuan, Wanzhe
    Wang, Jianchang
    Shipin Kexue/Food Science, 2020, 41 (04): : 268 - 272
  • [42] BK virus DNA detection by real-time polymerase chain reaction in clinical specimens
    Marchetti, Simona
    Graffeo, Rosalia
    Siddu, Alessia
    Santangelo, Rosaria
    Ciotti, Marco
    Picardi, Alessandra
    Favalli, Cartesio
    Fadda, Giovanni
    Cattani, Paola
    NEW MICROBIOLOGICA, 2007, 30 (02): : 119 - 126
  • [43] Detection of Leptospiral DNA by Real-time Polymerase Chain Reaction in Acute Weil Syndrome
    Gavin, Patrick
    Thomson, Richard, Jr.
    Donnersberger, David
    Fischer, Matthew
    Vescio, Thomas
    Levett, Paul
    INFECTIOUS DISEASES IN CLINICAL PRACTICE, 2005, 13 (04) : 187 - 189
  • [44] Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection
    Sun, Shipeng
    Meng, Shuang
    Zhang, Rui
    Zhang, Kuo
    Wang, Lunan
    Li, Jinming
    VIROLOGY JOURNAL, 2011, 8
  • [45] Investigation of presence of Cytomegalovirus DNA by real-time polymerase chain reaction in transplant recipients
    Fatma, Esenkaya Tathbent
    Mehmet, Ozdemir
    Bahadyr, Feyziodlu
    Metin, Dodan
    Udur, Tuzuner
    Mahmut, Baykan
    Mustafa, Altindis
    JOURNAL OF CLINICAL VIROLOGY, 2015, 70 : S105 - S105
  • [46] Detection of Histoplasma capsulatum DNA in human samples by real-time polymerase chain reaction
    Simon, Stephane
    Veron, Vincent
    Boukhari, Rachida
    Blanchet, Denis
    Aznar, Christine
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2010, 66 (03) : 268 - 273
  • [47] Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection
    Shipeng Sun
    Shuang Meng
    Rui Zhang
    Kuo Zhang
    Lunan Wang
    Jinming Li
    Virology Journal, 8
  • [48] Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction
    López-Andreo, M
    Lugo, L
    Garrido-Pertierra, A
    Prieto, MI
    Puyet, A
    ANALYTICAL BIOCHEMISTRY, 2005, 339 (01) : 73 - 82
  • [49] APPLICATION OF REAL-TIME POLYMERASE CHAIN REACTION FOR RAPID DETERMINATION OF SALMONELLA IN RESTAURANT FOODS
    Berrada, H.
    Soriano, J. M.
    Pico, Y.
    Manes, J.
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2008, 16 (04): : 299 - 307
  • [50] TaqMan real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
    Ali, Md. Eaqub
    Hashim, Uda
    Mustafa, Shuhaimi
    Man, Yaakob Bin Che
    Latif, Md. Abdul
    Islam, Khondakar Nurul
    Bakar, Md. Zuki Bin Abu
    Rahman, Md. Majibar
    JOURNAL OF FOOD AND NUTRITION RESEARCH, 2012, 51 (01): : 1 - 12
12345