Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction

被引：0
|
作者：
Slyadnev, M.N. [1 ,2 ]
Lavrova, M.V. [1 ,2 ]
Erkin, M.A. [1 ]
Kazakov, V.A. [2 ]
Ganeev, A.A. [1 ]
机构：
[1] Department of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets 198504, Russia
[2] NPF AP Lumex, Moskovskii pr. 19, St. Petersburg 190005, Russia
来源：
Journal of Analytical Chemistry | 2008年 / 63卷 / 02期
关键词：
A microfluidic system that allowed us to perform the real-time polymerase chain reaction (PCR) in a glass-silicon microchip containing nine 250-nL microreactors was developed and studied. The resulting high heating/cooling rates of a PCR mixture in a microreactor allowed us to optimize the amplification mode (1 min/cycle). The silicon surface of microreactors was successfully passivated. The resulting analytical system allowed us to measure the PCR kinetic curves in chip microreactors at a DNA concentration of ∼5 × 104 copies per microreactor. It was found that; if the PCR is performed in a microchip with real-time detection using the optimized amplification mode; the result can be obtained 13-14 min after the onset of reaction. © Pleiades Publishing; Ltd; 2008;
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页码：192 / 198
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