SEPARATION OF AMINO-ACID HOMOPOLYMERS BY CAPILLARY GEL-ELECTROPHORESIS

被引：36
|
作者：
DOLNIK, V [1 ]
NOVOTNY, MV [1 ]
机构：
[1] INDIANA UNIV, DEPT CHEM, BLOOMINGTON, IN 47405 USA
来源：
ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期
关键词：
D O I：
10.1021/ac00053a012
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Gel-filled capillaries utilizing highly concentrated and moderately cross-linked acrylamide-type gels in capillary electrophoresis were successfully applied to the separation of the individual oligomers of various poly(amino acids). Mixtures of both anionic and cationic nature were adequately resolved. While UV detection at 220 nm was mostly utilized, the polyanions with N-terminal groups can also be tagged with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) for a more sensitive detection by a laser-induced fluorescence detector.
引用
收藏
页码：563 / 567
页数：5
相关论文
共 50 条
  • [41] EFFECT OF UREA CONCENTRATION ON THE BASE-SPECIFIC SEPARATION OF OLIGODEOXYNUCLEOTIDES IN CAPILLARY AFFINITY GEL-ELECTROPHORESIS
    BABA, Y
    TSUHAKO, M
    SAWA, T
    AKASHI, M
    JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (01) : 93 - 99
  • [42] FAST PROTEIN SEPARATION BY CAPILLARY SDS GEL-ELECTROPHORESIS USING A UV TRANSPARENT POLYMER NETWORK
    SHIEH, P
    GUTTMAN, A
    HOANG, D
    COOKE, N
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 207 : 91 - ANYL
  • [43] CONTINUOUS IN-VIVO MONITORING OF AMINO-ACID NEUROTRANSMITTERS BY MICRODIALYSIS SAMPLING WITH ONLINE DERIVATIZATION AND CAPILLARY ELECTROPHORESIS SEPARATION
    ZHOU, SY
    ZUO, H
    STOBAUGH, JF
    LUNTE, CE
    LUNTE, SM
    ANALYTICAL CHEMISTRY, 1995, 67 (03) : 594 - 599
  • [44] Separation of hyaluronic acid by ultrahigh-voltage capillary gel electrophoresis
    Hutterer, KM
    Jorgenson, JW
    ELECTROPHORESIS, 2005, 26 (10) : 2027 - 2033
  • [45] PULSED-FIELD GEL-ELECTROPHORESIS ANALYSIS OF THE GENOME OF AMINO-ACID PRODUCING CORYNEBACTERIA - CHROMOSOME SIZES AND DIVERSITY OF RESTRICTION PATTERNS
    CORREIA, A
    MARTIN, JF
    CASTRO, JM
    MICROBIOLOGY-SGM, 1994, 140 : 2841 - 2847
  • [46] SEPARATION OF THYMUS HYPOCALCEMIC PRINCIPLE ON SDS GEL-ELECTROPHORESIS
    NARDELL, BE
    JOURNAL OF DENTAL RESEARCH, 1976, 55 : B77 - B77
  • [47] HUMAN STR ANALYSIS BY DYNAMIC SIEVING AND CAPILLARY GEL-ELECTROPHORESIS
    WILLIAMS, PE
    MARINO, MA
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 207 : 83 - ANYL
  • [48] INFLUENCE OF PH ON THE MIGRATION PROPERTIES OF OLIGONUCLEOTIDES IN CAPILLARY GEL-ELECTROPHORESIS
    GUTTMAN, A
    ARAI, A
    MAGYAR, K
    JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2): : 175 - 179
  • [49] ANALYSIS OF BOVINE IMMUNOGLOBULIN-G BY CAPILLARY GEL-ELECTROPHORESIS
    BENNETT, LE
    CHARMAN, WN
    WILLIAMS, DB
    CHARMAN, SA
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1994, 12 (09) : 1103 - 1108
  • [50] CAPILLARY SODIUM DODECYL-SULFATE GEL-ELECTROPHORESIS OF PROTEINS
    GUTTMAN, A
    NOLAN, JA
    COOKE, N
    JOURNAL OF CHROMATOGRAPHY, 1993, 632 (1-2): : 171 - 175
12345