SEPARATION OF AMINO-ACID HOMOPOLYMERS BY CAPILLARY GEL-ELECTROPHORESIS

被引：36
|
作者：
DOLNIK, V [1 ]
NOVOTNY, MV [1 ]
机构：
[1] INDIANA UNIV, DEPT CHEM, BLOOMINGTON, IN 47405 USA
来源：
ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期
关键词：
D O I：
10.1021/ac00053a012
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Gel-filled capillaries utilizing highly concentrated and moderately cross-linked acrylamide-type gels in capillary electrophoresis were successfully applied to the separation of the individual oligomers of various poly(amino acids). Mixtures of both anionic and cationic nature were adequately resolved. While UV detection at 220 nm was mostly utilized, the polyanions with N-terminal groups can also be tagged with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) for a more sensitive detection by a laser-induced fluorescence detector.
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页码：563 / 567
页数：5
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