SEPARATION OF AMINO-ACID HOMOPOLYMERS BY CAPILLARY GEL-ELECTROPHORESIS

被引：36
|
作者：
DOLNIK, V [1 ]
NOVOTNY, MV [1 ]
机构：
[1] INDIANA UNIV, DEPT CHEM, BLOOMINGTON, IN 47405 USA
来源：
ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期
关键词：
D O I：
10.1021/ac00053a012
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Gel-filled capillaries utilizing highly concentrated and moderately cross-linked acrylamide-type gels in capillary electrophoresis were successfully applied to the separation of the individual oligomers of various poly(amino acids). Mixtures of both anionic and cationic nature were adequately resolved. While UV detection at 220 nm was mostly utilized, the polyanions with N-terminal groups can also be tagged with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) for a more sensitive detection by a laser-induced fluorescence detector.
引用
收藏
页码：563 / 567
页数：5
相关论文
共 50 条
  • [31] CAPILLARY GEL-ELECTROPHORESIS AS A METHOD TO DETERMINE LIGATION EFFICIENCY
    COURTNEY, BC
    WILLIAMS, KC
    BING, QA
    SCHLAGER, JJ
    ANALYTICAL BIOCHEMISTRY, 1995, 228 (02) : 281 - 286
  • [32] PREDICTION OF MIGRATION BEHAVIOR OF OLIGONUCLEOTIDES IN CAPILLARY GEL-ELECTROPHORESIS
    GUTTMAN, A
    NELSON, RJ
    COOKE, N
    JOURNAL OF CHROMATOGRAPHY, 1992, 593 (1-2): : 297 - 303
  • [33] RESOLUTION AND CONCENTRATION DETECTION LIMIT IN CAPILLARY GEL-ELECTROPHORESIS
    MACEK, J
    TJADEN, UR
    VANDERGREEF, J
    JOURNAL OF CHROMATOGRAPHY, 1991, 545 (01): : 177 - 182
  • [34] ANALYSIS OF SYNTHETIC OLIGONUCLEOTIDE PURITY BY CAPILLARY GEL-ELECTROPHORESIS
    DUBROW, RS
    AMERICAN LABORATORY, 1991, 23 (05) : 64 - &
  • [35] ANALYSIS OF RESOLUTION IN DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS
    LUCKEY, JA
    NORRIS, TB
    SMITH, LM
    JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (12): : 3067 - 3075
  • [36] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY GEL-ELECTROPHORESIS AFTER TRYPTIC DIGESTION IN POLYACRYLAMIDE MATRIX
    ECKERSKORN, C
    LOTTSPEICH, F
    CHROMATOGRAPHIA, 1989, 28 (1-2) : 92 - 94
  • [37] CAPILLARY GEL-ELECTROPHORESIS AND THE ANALYSIS OF PHOSPHODIESTER AND PHOSPHOROTHIOATE DNA
    DEDIONISIO, L
    AMERICAN LABORATORY, 1994, 26 (11) : 30 - 34
  • [38] STABILITY MEASUREMENTS OF ANTISENSE OLIGONUCLEOTIDES BY CAPILLARY GEL-ELECTROPHORESIS
    BRUIN, GJM
    BORNSEN, KO
    HUSKEN, D
    GASSMANN, E
    WIDMER, HM
    PAULUS, A
    JOURNAL OF CHROMATOGRAPHY A, 1995, 709 (01) : 181 - 195
  • [39] DIFFUSION-COEFFICIENTS OF OLIGONUCLEOTIDES IN CAPILLARY GEL-ELECTROPHORESIS
    YIN, HF
    KLEEMISS, MH
    LUX, JA
    SCHOMBURG, G
    JOURNAL OF MICROCOLUMN SEPARATIONS, 1991, 3 (04) : 331 - 335
  • [40] SEPARATION OF LONG DNA FRAGMENTS BY CAPILLARY GEL-ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION
    NISHIKAWA, T
    KAMBARA, H
    ELECTROPHORESIS, 1994, 15 (02) : 215 - 220
12345