DISCRETE DOMAINS OF HUMAN U6 SNRNA REQUIRED FOR THE ASSEMBLY OF U4 U6 SNRNP AND SPLICING COMPLEXES

被引：42
|
作者：
BINDEREIF, A [1 ]
WOLFF, T [1 ]
GREEN, MR [1 ]
机构：
[1] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138
来源：
EMBO JOURNAL | 1990年 / 9卷 / 01期
关键词：
snRNA; splicing; U4/U6; snRNP;
D O I：
10.1002/j.1460-2075.1990.tb08102.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.
引用
收藏
页码：251 / 255
页数：5
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