DISCRETE DOMAINS OF HUMAN U6 SNRNA REQUIRED FOR THE ASSEMBLY OF U4 U6 SNRNP AND SPLICING COMPLEXES

被引：42
|
作者：
BINDEREIF, A [1 ]
WOLFF, T [1 ]
GREEN, MR [1 ]
机构：
[1] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138
来源：
EMBO JOURNAL | 1990年 / 9卷 / 01期
关键词：
snRNA; splicing; U4/U6; snRNP;
D O I：
10.1002/j.1460-2075.1990.tb08102.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.
引用
收藏
页码：251 / 255
页数：5
相关论文
共 50 条
  • [21] EVIDENCE FOR A PRP24 BINDING-SITE IN U6 SNRNA AND IN A PUTATIVE INTERMEDIATE IN THE ANNEALING OF U6 AND U4 SNRNAS
    JANDROSITZ, A
    GUTHRIE, C
    EMBO JOURNAL, 1995, 14 (04): : 820 - 832
  • [22] MULTIPLE ROLES FOR U6 SNRNA IN THE SPLICING PATHWAY
    MADHANI, HD
    BORDONNE, R
    GUTHRIE, C
    GENES & DEVELOPMENT, 1990, 4 (12B) : 2264 - 2277
  • [23] ANTISENSE PROBING OF THE HUMAN U4/U6 SNRNP WITH BIOTINYLATED 2'-OME RNA OLIGONUCLEOTIDES
    BLENCOWE, BJ
    SPROAT, BS
    RYDER, U
    BARABINO, S
    LAMOND, AI
    CELL, 1989, 59 (03) : 531 - 539
  • [24] CHARACTERIZATION OF A HUMAN U6 SNRNA GENE
    APPEL, B
    THEISSEN, H
    LUHRMANN, R
    RINKE, J
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1983, 364 (07): : 778 - 779
  • [25] Spliceosome assembly in the absence of stable U4/U6 RNA pairing
    Burke, Jordan E.
    Butcher, Samuel E.
    Brow, David A.
    RNA, 2015, 21 (05) : 923 - 934
  • [26] DHX15-independent roles for TFIP11 in U6 snRNA modification, U4/U6.U5 tri-snRNP assembly and pre-mRNA splicing fidelity
    Amandine Duchemin
    Tina O’Grady
    Sarah Hanache
    Agnès Mereau
    Marc Thiry
    Ludivine Wacheul
    Catherine Michaux
    Eric Perpète
    Eric Hervouet
    Paul Peixoto
    Felix G. M. Ernst
    Yann Audic
    Franck Dequiedt
    Denis L. J. Lafontaine
    Denis Mottet
    Nature Communications, 12
  • [27] DHX15-independent roles for TFIP11 in U6 snRNA modification, U4/U6.U5 tri-snRNP assembly and pre-mRNA splicing fidelity
    Duchemin, Amandine
    O'Grady, Tina
    Hanache, Sarah
    Mereau, Agnes
    Thiry, Marc
    Wacheul, Ludivine
    Michaux, Catherine
    Perpete, Eric
    Hervouet, Eric
    Peixoto, Paul
    Ernst, Felix G. M.
    Audic, Yann
    Dequiedt, Franck
    Lafontaine, Denis L. J.
    Mottet, Denis
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [28] Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 tri-snRNP as revealed by electron cryomicroscopy
    Sander, Bjoern
    Golas, Monika M.
    Makarov, Evgeny M.
    Brahms, Hero
    Kastner, Berthold
    Luehrmann, Reinhard
    Stark, Holger
    MOLECULAR CELL, 2006, 24 (02) : 267 - 278
  • [29] U1, U2, AND U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEINS ARE REQUIRED FOR INVITRO SPLICING BUT NOT POLYADENYLATION
    BERGET, SM
    ROBBERSON, BL
    CELL, 1986, 46 (05) : 691 - 696
  • [30] U6 snRNA maturation and stability
    Fury, MG
    Zieve, GW
    EXPERIMENTAL CELL RESEARCH, 1996, 228 (01) : 160 - 163
12345