DISCRETE DOMAINS OF HUMAN U6 SNRNA REQUIRED FOR THE ASSEMBLY OF U4 U6 SNRNP AND SPLICING COMPLEXES

被引：42
|
作者：
BINDEREIF, A [1 ]
WOLFF, T [1 ]
GREEN, MR [1 ]
机构：
[1] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138
来源：
EMBO JOURNAL | 1990年 / 9卷 / 01期
关键词：
snRNA; splicing; U4/U6; snRNP;
D O I：
10.1002/j.1460-2075.1990.tb08102.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.
引用
收藏
页码：251 / 255
页数：5
相关论文
共 50 条
  • [11] Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
    Montemayor, Eric J.
    Didychuk, Allison L.
    Yake, Allyson D.
    Sidhu, Gurnimrat K.
    Brow, David A.
    Butcher, Samuel E.
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2018, 74 : A359 - A359
  • [12] Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
    Montemayor, Eric J.
    Didychuk, Allison L.
    Yake, Allyson D.
    Sidhu, Gurnimrat K.
    Brow, David A.
    Butcher, Samuel E.
    NATURE COMMUNICATIONS, 2018, 9
  • [13] Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
    Eric J. Montemayor
    Allison L. Didychuk
    Allyson D. Yake
    Gurnimrat K. Sidhu
    David A. Brow
    Samuel E. Butcher
    Nature Communications, 9
  • [14] All snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli; Identification of the NoLE of U6 snRNA
    Gerbi, SA
    Borovjagin, AV
    Lange, TS
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 140A - 140A
  • [15] CONSERVED DOMAINS OF HUMAN U4 SNRNA REQUIRED FOR SNRNP AND SPLICEOSOME ASSEMBLY
    WERSIG, C
    BINDEREIF, A
    NUCLEIC ACIDS RESEARCH, 1990, 18 (21) : 6223 - 6229
  • [16] ELECTRON-MICROSCOPY OF U4/U6 SNRNP REVEALS A Y-SHAPED U4 AND U6 RNA CONTAINING DOMAIN PROTRUDING FROM THE U4 CORE RNP
    KASTNER, B
    BACH, M
    LUHRMANN, R
    JOURNAL OF CELL BIOLOGY, 1991, 112 (06): : 1065 - 1072
  • [17] GENETIC DISSECTION OF THE U4/U6/U5 SNRNP CYCLE IN YEAST
    GUTHRIE, C
    SHANNON, K
    BORDONNE, R
    MOLECULAR BIOLOGY REPORTS, 1990, 14 (2-3) : 139 - 140
  • [18] Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET
    Hardin, John W.
    Warnasooriya, Chandani
    Kondo, Yasushi
    Nagai, Kiyoshi
    Rueda, David
    NUCLEIC ACIDS RESEARCH, 2015, 43 (22) : 10963 - 10974
  • [19] SUPPRESSORS OF A U4 SNRNA MUTATION DEFINE A NOVEL U6 SNRNP PROTEIN WITH RNA-BINDING MOTIFS
    SHANNON, KW
    GUTHRIE, C
    GENES & DEVELOPMENT, 1991, 5 (05) : 773 - 785
  • [20] INVITRO RECONSTITUTION OF SNRNPS - A RECONSTITUTED U4/U6 SNRNP PARTICIPATES IN SPLICING COMPLEX-FORMATION
    PIKIELNY, CW
    BINDEREIF, A
    GREEN, MR
    GENES & DEVELOPMENT, 1989, 3 (04) : 479 - 487
12345