IMPROVEMENT OF ONLINE DETECTION IN HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY - UV ABSORPTIOMETRY AND EVAPORATIVE LIGHT-SCATTERING DETECTION

Improvements in analytical high-speed counter-current chromatography involve reduction of the column volume and a high revolution speed in order to decrease the time of analysis. Continuous detection should be performed instead of laborious fraction collection before reading the UV absorbance of the fractions. In this work on-line UV absorptiometry was performed by adding a "make-up" solvent to the column effluent in order to obtain a homogeneous medium before the detection is carried out. The first attempt to use an evaporative light-scattering detector is discussed.