ENZYMATIC ASSAY FOR ADENOSYLCOBALAMIN-DEPENDENT METHYLMALONYL COENZYME-A MUTASE

被引：8

作者：

WATANABE, F ^{[1
]}

TAMURA, Y ^{[1
]}

SAIDO, H ^{[1
]}

NAKANO, Y ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE, DEPT AGR CHEM, SAKAI, OSAKA 593, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期

关键词：

D O I：

10.1271/bbb.57.1593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzymatic method for assaying methylmalonyl-CoA mutase was devised. The method employs a succinyl-CoA transferase and beta-hydroxyacyl-CoA dehydrogenase-coupled reaction to make the formation of NAD+ stoichiometric with the amount of the succinyl-CoA formed by the mutase. This method is one of the most convenient and useful assays for the activity of methylmalonyl-CoA mutase in biological samples.

引用

页码：1593 / 1594

页数：2

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